摘要
为探明朱砂根种胚离体快繁的适宜培养条件,以不同胚龄朱砂根种子为试验材料,开展朱砂根种胚离体快繁生长成苗调控技术研究。结果表明,朱砂根种胚在1/2 MS培养基中萌发快,培养5 d萌发率达100%,显著高于MS和WPM培养基(p<0.05);种胚萌发率随胚龄增加先升后降;大量元素水平适当减少有利于幼苗生长,于1/4 MS培养基中生长的长势最佳;相关性分析表明,移栽存活率与幼苗根条数呈显著正相关(p<0.05),与幼苗茎粗呈极显著负相关(p<0.01);正交试验结果表明,适宜的蔗糖浓度、基本培养基、植物激素配比,可获得根条数多,且根粗和茎粗适中的朱砂根理想株型,提高移栽成活率。因此,朱砂根种胚繁育技术为:47 d胚龄种胚,接种于1/2 MS培养基中萌发,继代于1/4 MS培养基中生长,于1/2 MS+0.5 mg/L 6-BA+2.5 mg/L NAA+20 g/L蔗糖(pH=5.8)培养基质中壮苗,移栽于质量比为1∶1(育苗基质∶腐殖土)的基质中。
To explore the suitable culture conditions for rapid propagation of Ardisia crenata Sims embryos in vitro, the seeds of A. crenata with different maturity ages were selected as experimental materials, the regulation technology of rapid propagation and the growth of A. crenata embryos in vitro were studied. The results showed that the high germination rate of A. crenata embryos in 1/2 MS medium, was 100% after 5 days, which was significantly higher than that in MS and WPM medium(p<0.05). The germination rate of embryo increased firstly and then decreased with the increase of embryo age, proper reduction of large elements was beneficial to seedling growth, which had the best growth in 1/4 MS medium;Correlation analysis showed that transplanting survival rate was significantly positively correlated with the number of roots of seedlings(p<0.05), and was significantly negatively correlated with stem diameter of seedlings(p<0.01). Orthogonal test results showed that the optimum sucrose concentration, basic medium and plant hormone ratio could obtain the ideal plant type of with more roots and moderate root and stem diameter, and improve the survival rate of transplanting. Therefore, the regulation technology of A. crenata Sims embryo was embryo aged 47 days were incubated in 1/2 MS medium for germination and subcultured in 1/4 MS medium for growth. Strong seedlings in 1/2 MS medium with 0.5 mg/L 6-BA+2.5 mg/L NAA+20 g/L sucrose(pH=5.8), and transplanted in a mass ratio of 1∶1(nursery substrates to humus soil).
作者
周琪
吕享
林冰
田小桥
钟双萍
李佩仪
ZHOU Qi;L Xiang;LIN Bing;TIAN Xiaoqiao;ZHONG Shuangping;LI Peiyi(College of Pharmaceutical Sciences,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Food Safety Quality Control Engineering Technology of Guizhou University of Traditional Chinese Medicine Research Center,Guiyang 550025,China)
出处
《种子》
北大核心
2022年第8期104-109,115,共7页
Seed
基金
国家重点研发计划(2019 YFC 1712503)
贵州省特色功能食品与中药制剂开发攻关大平台(黔教合KY字[2020]006)
贵中医博士启动([2019]70号)。
关键词
朱砂根
种胚繁育
离体培养
Ardisia crenata Sims
embryo breeding
rapid propagation in vitro
