摘要
目的:研究余甘子总酚对载脂蛋白E基因敲除(ApoE^(-/-))动脉粥样硬化模型小鼠的肝脏保护作用,初步探讨其作用机制。方法:建立高脂饮食诱导的ApoE^(-/-)小鼠动脉粥样硬化模型,给予余甘子总酚灌胃8周后,酶联免疫法检测小鼠血清中天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)水平;观察小鼠肝脏病理模型切片;酶联免疫法检测小鼠肝脏组织匀浆中的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、白细胞介素6(IL-6)、IL-1β和肿瘤坏死因子α(TNF-α)的水平;Western blot法检测小鼠肝脏组织相关蛋白的表达。结果:余甘子总酚干预可降低ApoE^(-/-)小鼠血清中AST和ALT水平(P<0.05),减缓小鼠肝脏脂肪性病变及炎症细胞浸润现象;同时,余甘子总酚可显著降低肝脏组织匀浆中TC、TG和LDL-C水平,提高HDL-C水平(P<0.05),显著降低炎症因子IL-6、IL-1β和TNF-α含量(P<0.05);此外,余甘子总酚还可显著升高模型小鼠肝脏组织ATP结合盒转运体A1(ABCA1)蛋白水平(P<0.05),降低小鼠肝脏组织清道夫受体CD36和类固醇受体RNA激活物1(SRA1),以及Toll样受体4(TLR4)和核因子κB(NF-κB)P65蛋白表达水平(P<0.05)。结论:余甘子总酚可减少肝脏内胆固醇蓄积和炎症因子的释放,从而减轻动脉粥样硬化小鼠肝脏脂肪性病变和炎症反应。
AIM:To study the liver protective effect and mechanism of total phenols from Phyllanthus emblica L.(PE)in ApoE^(-/-)atherosclerosis mice.METHODS:The atherosclerosis ApoE^(-/-)mice were induced by a high-fat diet for 8 weeks. The levels of aspartate transaminase(AST)and alanine aminotransferase(ALT)in serum of mice were detected by ELISA. Meanwhile,the pathological sections of liver of mice were observed. The levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),interleukin-6(IL-6),IL-1β,and tumor necrosis factor-α(TNF-α)in mouse liver tissue homogenate were measured by enzyme-linked immunoassay. The expression of related proteins in liver tissues of mice was detected by Western blot.RESULTS:Serum AST and ALT levels in ApoE^(-/-)mice were decreased by PE intervention(P<0. 05). Lipid deposition and inflammatory factors were alleviated. The contents of TC,TG and LDL-C in liver homogenate were significantly decreased(P<0. 05),while the content of HDL-C was increased(P<0. 05). Meanwhile,the levels of IL-6,IL-1β and TNF-α were obviously suppressed by PE(P<0. 05). In addition,the protein level of ATP binding cassette transporter A1(ABCA1)in liver tissue of mice was significantly increased(P<0. 05),while the levels of CD36 and steroid receptor RNA activator 1(SRA1)in liver tissues were reduced. Moreover,the protein expression of Toll-like receptor 4(TLR4)and nuclear factor-κB(NF-κB)P65 were decreased(P<0. 05).CONCLUSION:Treatment with PE reduces the accumulation of cholesterol and the release of inflammatory factors in the liver,thus attenuating the fatty deposition and inflammation in the liver of atherosclerotic mice.
作者
邓欣
孟达
吴静宜
姜丙通
张雅琼
赵毅
车彦云
DENG Xin;MENG Da;WU Jing-yi;JIANG Bing-tong;ZHANG Ya-qiong;ZHAO Yi;CHE Yan-yun(Engineering Laboratory for National Healthcare Theories and Products of Yunnan Province,Yunnan University of Chinese Medicine,Kunming 650500,China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2022年第9期1608-1617,共10页
Chinese Journal of Pathophysiology
基金
云南省科技厅-云南中医药大学应用基础联合研究专项项目重点项目(No.2019FF002-012)
云南省科技厅-云南中医药大学应用基础联合研究专项项目面上项目(No.202101AZ070001-212)。
关键词
余甘子总酚
动脉粥样硬化
肝
脂质代谢
炎症
Total phenols from Phyllanthus emblica L.
Atherosclerosis
Liver
Lipid metabolism
Inflammation
