摘要
目的建立同时测定醒脾养儿颗粒中新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C 6种咖啡酰奎宁酸类成分含量的高效液相色谱法。方法色谱柱为ACE Excel 5 C柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.2%磷酸水溶液(梯度洗脱),流速为1.0 mL/min,检测波长为325 nm,柱温为30℃,进样量为10μL。结果新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C质量浓度分别在2.65~53.06μg/mL、4.20~84.00μg/mL、3.43~68.57μg/mL、1.40~27.96μg/mL、0.72~14.47μg/mL、2.06~41.45μg/mL范围内与峰面积线性关系良好(r≥0.9996,n=6);精密度、稳定性、重复性试验结果的RSD均低于2.0%(n=6);平均加样回收率分别为100.48%,99.34%,102.95%,103.13%,102.03%,103.02%,RSD分别为1.09%,0.73%,1.71%,2.36%,2.43%,0.53%(n=6)。21批样品中6种咖啡酰奎宁酸类成分的总含量为0.30291~0.68319 mg/g,平均含量为0.48599 mg/g。结论该方法操作简便、重复性好,可用于醒脾养儿颗粒中6种咖啡酰奎宁酸类成分的含量测定。
Objective To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of six caffeoylquinic acids including neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in Xingpi Yang’er Granules.Methods The chromatographic column was ACE Excel 5 Ccolumn(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.2%phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 325 nm,the column temperature was 30℃,and the injection volume was 10μL.Results The linear ranges of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C were 2.65-53.06μg/mL,4.20-84.00μg/mL,3.43-68.57μg/mL,1.40-27.96μg/mL,0.72-14.47μg/mL and 2.06-41.45μg/mL(r≥0.9996,n=6),respectively.The RSDs of precision,stability and repeatability tests were lower than 2.0%(n=6).The average recoveries of the above six caffeoylquinic acids were 100.48%,99.34%,102.95%,103.13%,102.03%and 103.02%with RSDs of 1.09%,0.73%,1.71%,2.36%,2.43%,and 0.53%(n=6),respectively.The total content of six caffeoylquinic acids in the 21 batches of samples was in the range of0.30291-0.68319 mg/g,with the average content of 0.48599 mg/g.Conclusion The method is simple and repeatable,which can be used for the determination of six caffeoylquinic acids in Xingpi Yang’er Granules.
作者
朱帮会
李银
马雪
汪洋
刘春花
田琳
李勇军
ZHU Banghui;LI Yin;MA Xue;WANG Yang;LIU Chunhua;TIAN Lin;LI Yongjun(Engineering Research Center for the Development and Application of Ethnic Medicine and TCM<Ministry of Education>,Guizhou Medical University·State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang,Guizhou,China 550004;School of Pharmacy,Guizhou Medical University,Guiyang,Guizhou,China 550004;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang,Guizhou,China 550004;Guizhou Jianxing Pharmaceutical Co.,Ltd.,Guiyang,Guizhou,China 550018)
出处
《中国药业》
CAS
2022年第19期59-63,共5页
China Pharmaceuticals
基金
贵州省科技计划项目[黔科合支撑[2019]2779号,黔科合平台人才[2016]5677]。
