摘要
【目的】探索鸡矢藤有效活性成分的体外抑菌效果、体内抗炎镇痛作用,为充分利用鸡矢藤资源提供理论依据。【方法】用40%乙醇溶液提取鸡矢藤,对所得提取物进行化学成分预试分析;采用药敏试验和二倍梯度稀释法检测其对大肠杆菌、沙门氏菌、金黄色葡萄球菌和蜡状芽孢杆菌的抑菌活性,测定MIC;探究鸡矢藤对二甲苯所致小鼠耳廓肿胀、角叉莱胶所致小鼠足跖肿胀的抗炎作用;并研究鸡矢藤对冰醋酸所致小鼠扭体疼痛的镇痛作用。【结果】鸡矢藤乙醇提取液含有黄酮、萜类内酯等多种有效化学成分;能够抑制蜡状芽胞杆菌生长,抑菌圈直径为8.55 mm,MIC为500 mg/mL,但对大肠杆菌、沙门氏菌和金黄色葡萄球菌的抑菌效果不明显;与对照处理相比,中、高剂量鸡矢藤提取液对二甲苯所致小鼠耳廓炎性肿胀的抑制率分别为30.93%和55.00%,对角叉莱胶所致小鼠足跖肿胀的抑制率分别为49.74%和58.26%,抗炎作用极显著,且能够有效缓减冰醋酸引起的小鼠外周性疼痛,使小鼠首次出现扭体反应的时间延迟,15 min内出现扭体反应的次数减少。【结论】鸡矢藤乙醇提取液含有丰富的药效活性成分,体外抑菌效果较弱,但对试验小鼠抗炎和镇痛作用显著,可作为畜禽饲料添加剂候选中草药资源。
【Objective】The antibacterial effect in vitro and the anti-inflammatory and analgesic effects in vivo of the effective active components of Paederia scanden were explored in order to provide theoretical support for the full utilization of P.scanden resources.【Method】The P.scanden was extracted with 40% ethanol solution,and the chemical components of the extract were preliminarily analyzed.The antimicrobial activities of the extract to Escherichia coli,Salmonella,Staphylococcus aureus and Bacillus cereus were detected by drug sensitivity test and double gradient dilution method to determine the minimum inhibitory concentration(MIC).The anti-inflammatory effect of P.scanden was explored by relieving xylene-induced ear swelling in mice and carrageenan-induced mice foot swelling.And the analgesic effect of acetic acid-induced writhing pain in mice was studied.【Result】The ethanol extract of P.scanden contained many effective chemical components such as flavonoids and terpene lactones,which could inhibit the growth of B.cereus,with the inhibitory zone diameter of 8.55 mm and MIC of 500 mg/mL,but the antibacterial effect on E.coli,Salmonella and S.aureus was not obvious.Compared with the control group,the inhibitory rates of medium and high doses of P.scanden extract on mice ear swelling induced by xylene were 30.93% and 55.00%,and the inhibition rates of mice foot swelling induced by carrageenan were 49.74% and 58.26%,respectively,showing extremely significant anti-inflammatory effects,and they could effectively alleviate peripheral pain caused by glacial acetic acid in mice,delaying the time of the first writhing reaction in mice and reducing the frequency of writhing reaction within 15 minutes.【Conclusion】The ethanol extract of P.scanden contains rich medicinal activity.Although the antibacterial effect in vitro is weak,the anti-inflammatory and analgesic effects on tested mice are remarkable.Therefore,it can be used as one of the candidate Chinese herbal resources for animal feed additives.
作者
杨宁宇
江佳丽
梁淑荧
曾琳
林红英
陈进军
康丹菊
YANG Ningyu;JIANG Jiali;LIANG Shuying;ZENG Lin;LIN Hongying;CHEN Jinjun;KANG Danju(College of Coastal Agricultural Sciences,Guangdong Ocean University,Zhanjiang 524088,China)
出处
《广东农业科学》
CAS
2022年第8期103-110,共8页
Guangdong Agricultural Sciences
基金
广东海洋大学博士启动基金(R20045)
2020年广东省一流专业-动物医学专项(010306052102)
广东省研究生教育创新计划项目-联合培养研究生示范基地专项(230420081)。
关键词
中草药
鸡矢藤
乙醇提取法
化学成分预试
抑菌作用
抗炎
镇痛
Chinese medicinal herb
Paederia scandens(Lour.)Merr
ethanol extraction method
chemical component pretest
antibacterial effect
anti-inflammatory
analgesic
