摘要
目的:通过比较不同模型建立方法,筛选制备高效、可靠的胆囊胆固醇结石动物模型。方法:将36只5周龄SPF级雄性ICR小鼠随机分为对照组、2%高脂组、1%胆固醇组,每组各12只。对照组采用普通颗粒饲料喂养,2%高脂组采用2%高脂饲料喂养,1%胆固醇组采用1%胆固醇饲料喂养。喂养16周,记录各组小鼠的死亡数量,计算存活率;观察并记录各组造模成功的小鼠数量及胆囊体积大小、胆汁的浑浊度、透光性等,计算小鼠成石率;ELISA法检测胆囊胆汁总胆固醇(total cholesterol,TC)、总胆汁酸(total bile acids,TBA)、磷脂(phospholipid,PL)及胆囊收缩素(cholecystokinin,CCK)的含量;HE染色观察小鼠胆囊、肝脏病理学变化。结果:对照组小鼠无死亡,胆囊大小正常,胆囊壁结构正常,胆汁澄清透亮,透光性好,胆囊内无沉淀;肝脏细胞排列整齐,结构完整。2%高脂组小鼠死亡1只,胆囊壁肌层变厚,浆膜结构及形态破坏,胆汁呈浑浊状态,透光性差,成石率为66.67%;肝脏细胞结构破坏严重,可见大量脂质空泡,肝脏存在明显的脂肪变性。1%胆固醇组小鼠死亡1只,胆囊体积明显增大,胆囊壁肌层毛糙、增厚,浆膜结构及形态破坏严重,胆汁呈褐色浑浊状态,透光性差,成石率约为83.33%;肝细胞排列紊乱,细胞结构破坏,肝脏出现小的脂质空泡。与对照组比较,2%高脂组和1%胆固醇组胆汁内TC含量升高,胆汁TBA、PL、CCK含量降低;与2%高脂组比较,1%胆固醇组胆汁TC、CCK含量降低,胆汁内TB、PL含量升高,差异均有统计学意义(P<0.01)。结论:1%胆固醇饲料喂养建立胆囊胆固醇结石动物模型效果最好,可作为胆囊胆固醇结石相关研究的备选模型。
Objective:To screen the methods of establishing high efficient and reliable animal models of cholesterol gallstone by comparing different methods of establishing models.Methods:A total of 36 5-week-old male ICR mice of SPF grade were randomly divided into the control group,the 2% high fat group and the 1% cholesterol group,with 12 mice in each group.The control group was fed with ordinary pellet feed,the 2% high fat group was fed with 2% high fat feed,and the 1% cholesterol group was fed with 1% cholesterol feed.After feeding for 16 weeks,the number of dead mice in each group was recorded and the survival rate was calculated.The number of successful mice in each group,the size of gallbladder,the turbidity and transmittance of bile were observed and recorded,and the stone formation rate of mice was calculated.The contents of total cholesterol(TC),total bile acids(TBA),phospholipid(PL) and cholecystokinin(CCK) in gallbladder bile were detected by ELISA.The pathological changes of gallbladder and liver were observed by HE staining.Results:In the control group,the mice did not die,the size of the gallbladder was normal,the structure of the gallbladder wall was normal,the bile was clear and transparent,the light transmission was good,and there was no precipitation in the gallbladder.The liver cells were arranged in order with complete structure.In the 2% high fat group,one mouse died,the muscle layer of the gallbladder wall became thicker,the serosal structure and morphology were destroyed,the bile was turbid,the permeability was poor,and the stone formation rate was 66.67%.The cellular structure of the liver was severely damaged,a large number of lipid vacuoles could be seen,and there was obvious steatosis in the liver.In the 1% cholesterol group,one mouse died,the gallbladder volume increased significantly,the muscle layer of the gallbladder wall was rough and thickened,the serosal structure and morphology were seriously damaged,the bile was brown and turbid,the light transmittance was poor,and the stone formation rate was about 83.33%.The arrangement of hepatocytes was disordered,the cell structure was destroyed,and small lipid vacuoles appeared in the liver.Compared with the control group,the content of TC in the bile of the 2% high fat group and the 1% cholesterol group increased,while the contents of TBA,PL and CCK in the bile decreased.Compared with the 2% high fat group,the contents of TC and CCK in the bile of the 1% cholesterol group decreased,and the contents of TB and PL in the bile increased,and all the differences were statistically significant(P<0.01).Conclusion:The animal model of cholesterol gallstone established by feeding with 1% cholesterol diet is the best,and can be used as an alternative model for the study of cholesterol gallstone.
作者
谢玉春
闵莉
XIE Yu-chun;MIN Li(Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian,China,350122)
出处
《河南中医》
2022年第11期1671-1675,共5页
Henan Traditional Chinese Medicine
基金
福建省自然科学基金项目(2021J01928)。
