摘要
分析苦荞(Fagopyrum tataricum)种质资源遗传多样性和群体遗传结构,筛选与苦荞农艺性状关联的分子标记,为苦荞杂交组合的亲本选配及分子标记辅助育种提供依据。本试验以318份苦荞种质资源为材料,分别于2019年和2020年对其主要农艺性状(株高、主茎分枝数、主茎节数和千粒重)及籽粒特征值(粒长、粒宽、籽粒长宽比、籽粒面积、籽粒周长、籽粒直径、籽粒圆度)进行了表型鉴定,并通过分析2年的表型数据计算出BLUP值。77对具有良好多态性的SSR标记扫描群体,共检测到293个等位变异,基因多样性均值为0.52,PIC值平均为0.46;聚类分析将318份苦荞资源分成4大类,平均遗传距离0.44;群体结构分析表明该群体可分为2个亚群。上述SSR标记分别对这11个表型性状进行了关联分析,共检测有54个SSR标记与苦荞种质资源农艺性状极显著关联,表型变异解释率为1.77%~16.40%,其中25个标记在3个环境中均检测到,47个SSR标记同时和2个或以上的表型性状呈极显著关联。该研究结果对苦荞相关性状的候选基因挖掘和高产苦荞分子标记辅助育种具有重要意义。
The analysis of the genetic diversity and population genetic structure of Fagopyrum tataricum germplasm resources and the molecular markers associated with the agronomic traits and seed-related traits of Fagopyrum tataricum could provide a theoretical basis and reference for the hybrid combinations and molecular marker assisted breeding of Tartary buckwheat.In this study,318 Tartary buckwheat germplasms were used as materials.A total of 11 traits,including thousand-grain weight(TGW),grain length(GL),grain width(GW),grain length-to-width ratio(L/W),grain area(GA),grain perimeter(GP),grain diameter(GD),roundness of grain(GR),plant height(PH),branch number of main stem(BN),and the node pod number of main stem(PN),were investigated in two years of 2019–2020,and the phenotypic data were analyzed by BLUP.A total of 293 loci were detected by 77 SSR markers with good polymorphism in the tested Tartary buckwheat accessions.The average gene diversity coefficient was 0.52,and the average PIC value was 0.46.318 Tartary buckwheat resources were divided into 4 groups with an average genetic distance of 0.44 by cluster analysis and it had no obvious correspondence with geographical origin.Genetic structure analysis revealed that the tested group could be divided into two subgroups.There were detected extremely 54 significant loci at P<0.01 associated with 11 traits with the explanation rate ranging from 1.77%to 16.40%by association study.Among these markers,47SSRmarker could be detected by two or more traits at the same time and 25SSRmarker could be detected under 2019,2020,and BLUP,simultaneously.These results will be of great significance for candidate gene mining for related traits and molecular marker-assisted breeding of high-yield Tartary buckwheat.
作者
李晓瑜
方小梅
伍浩天
王莹倩
刘洋
唐恬
王于栋
吴银环
岳林清
张瑞丰
崔静斌
张建
易泽林
LI Xiao-Yu;FANG Xiao-Mei;WU Hao-Tian;WANG Ying-Qian;LIU Yang;TANG Tian;WANG Yu-Dong;WU Yin-Huan;YUE Lin-Qing;ZHANG Rui-Feng;CUI Jing-Bin;ZHANG Jian;YI Ze-Lin(College of Agronomy and Biotechnology,Southwest University/Chongqing Buckwheat Industry System Innovation Team,Chongqing 400715,China)
出处
《作物学报》
CAS
CSCD
北大核心
2022年第12期3091-3107,共17页
Acta Agronomica Sinica
基金
中国博士后科学基金面上项目(2017M622944)
重庆市荞麦技术产业体系建设项目(20170249)
重庆市自然科学基金项目(cstc2018jcyj AX0394)资助。
关键词
苦荞
农艺性状
籽粒性状
SSR标记
关联分析
Tartary buckwheat
agronomic traits
seed-related traits
SSR markers
association analysis
