摘要
油茶是一种潜在的经济林模式树种,其研究受到越来越多的重视。以油茶主栽品种‘华硕’为研究材料,通过RT-PCR克隆方法,获得一个高等植物特异的CC型谷氧还蛋白(glutaredoxin,GRX)基因家族成员,命名为Co ROXY19。Co ROXY19基因的开放阅读框(ORF)为444 bp,编码147个氨基酸,分子质量为15.62 kDa,具有典型的谷氧还蛋白结构域,包括酶活性中心、谷胱甘肽结合位点和转录因子结合位点。进化树分析发现CoROXY19与茶树CsROXY19同源关系最近,亚细胞定位发现CoROXY19蛋白在细胞质和细胞核中均有分布,酵母双杂交和双分子荧光互补实验证实CoROXY19与转录因子AtTGA2相互作用,实时荧光定量PCR表明CoROXY19基因在叶片中的表达受机械损伤诱导,预示油茶CoROXY19可能通过与转录因子相互作用,参与调节抗逆相关激素信号转导。
As a potential model tree species,Camellia oleifera has attracted increasing attention from the non-timber forest researchers.A higher plant-specific CC-type glutaredoxin(GRX) gene family member named CoROXY19 was isolated from a cultivar ‘Huashuo’.The open reading frame(ORF) of CoROXY19 is 444 bp,encoding 147 amino acids,and has a molecular mass of 15.62 kDa.It contains several typical glutaredoxin motifs,including an enzyme active center,glutathione binding site and transcriptional protein binding sites.Phylogenetic tree analysis showed that CoROXY19 has a close homology relationship with CsROXY19 from tea plant.Subcellular localization revealed that CoROXY19 protein were distributed in both cytoplasm and nucleus.The two-hybrid experiment confirmed that CoROXY19 interacted with AtTGA2.Real-time quantitative PCR showed that the expression of CoROXY19 was strongly induced by wounding stress.These experimental results suggest that CoROXY19 may be involved in regulating stress-related hormone signal transduction through interacting with transcription factors.
作者
梁心仪
严冰冰
林增
陈嘉丽
李宁
禹佩瑶
汤阳灿
雒建军
黄黎君
LIANG Xinyi;YAN Bingbing;LIN Zeng;CHEN Jiali;LI Ning;YU Peiyao;TANG Yangcan;LUO Jianjun;HUANG Lijun(College of Forestry,Key Laboratory of Cultivation and Protection for Non-wood Forest Trees,Central South University of Forestry and Technology,Changsha 410004,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2022年第8期1465-1474,共10页
Plant Physiology Journal
基金
长沙市杰出创新青年培养计划项目(kq2009016)
湖南省自然科学基金(2020JJ5970和2021JJ31141)
湖南省研究生科研创新项目(CX20210885)
湖南省大学生创新创业训练计划项目(S202010538004)。
关键词
油茶
经济林
谷氧还蛋白
抗逆激素
Camellia oleifera
non-timber forest
glutaredoxin
defense hormone
