摘要
苦荞Fagopyrum tataricum是四川省凉山彝族自治州主要的粮食作物之一,作为近年来新兴的保健食品,其种子携带的细菌种群鲜有报道。本研究从凉山州7个县的苦荞主产区共采集了46份苦荞种子样品,通过分离培养法及基于16S rRNA基因的扩增子测序法,对苦荞种子携带的主要细菌类群进行了研究。结果显示:分离培养法从46份样品中共获得618株细菌分离物,隶属于10个属,其中泛菌属Pantoea和短小杆菌属Curtobacterium分离频率最高,分别为52.5%和20.7%,是种子携带的优势菌群。扩增子测序法从13份样品中共获得279个属的细菌,其中泛菌属为优势菌群。通过样本层级聚类分析,发现来自冕宁县的苦荞种子样品MNCQ-4携带的细菌丰富度与多样性最大,而来自昭觉县的样品ZJ-5最小。研究结果表明,同一个地区的不同样品,菌群多样性也存在差异。本研究丰富了荞麦种带细菌的研究,为种子处理、病害防治提供了依据。
The tartary buckwheat,Fagopyrum tataricum,is one of the staple food crops in Liangshan Yi autonomous prefecture,Sichuan province.As a new healthy food in recent years,there have been few reports on the bacterial population carried by tartary buckwheat seeds.Here,46 tartary buckwheat seed samples were collected from the main tartary buckwheat producing areas in seven counties of Liangshan prefecture.The main bacterial populations carried by tartary buckwheat seeds were studied by agar plating and using 16 S rRNA amplicon sequencing method.The results showed that a total of 618 bacterial isolates belonging to 10 genera were obtained from 46 seeds samples by agar plating.Among them,Pantoea and Curtobacterium had the highest isolation frequency(52.5% and 20.7%,respectively),which were the dominant microflora carried by seeds.A total of 279 genera were identified from the 13 tested seed samples by 16 S rRNA amplicon sequencing method,and the dominant genus was Pantoea.Through sample hierarchy clustering analysis,it was found that MNCQ-4 from Mianning county had the highest community richness and diversity,while ZJ-5 from Zhaojue county had the lowest community richness and diversity.Moreover,the results showed that samples from the same area had different bacterial diversities.This study could enrich our understanding of buckwheat diseases and provide a reference for seed treatment and disease control.
作者
白凯红
阿别小兵
陈星
蒋娜
李健强
罗来鑫
BAI Kaihong;A’BIE Xiaobing;CHEN Xing;JIANG Na;LI Jianqiang;LUO Laixin(Department of Plant Pathology,China Agricultural University,Key Laboratory of Pest Monitoring and Green Management,Ministry of Agriculture and Rural Affairs,Beijing Key Laboratory of Seed Disease Testing and Control,Beijing 100193,China)
出处
《植物保护》
CAS
CSCD
北大核心
2022年第5期190-196,238,共8页
Plant Protection
基金
国家重点研发计划(2017YFD0201601)。
关键词
苦荞种子
细菌
分离鉴定
扩增子测序
tartary buckwheat seed
bacteria
isolation and identification
amplicon sequencing
