摘要
高浓度染毒水平全氟辛烷磺酸(PFOS)对植物体的毒性效应信息,不能作为低浓度环境水平毒性效应的依据,为了更好地了解不同浓度PFOS对植物体的毒性效应,本研究采用高通量非靶向代谢组学技术分析了不同浓度PFOS对拟南芥在代谢水平上的毒性效应。通过染毒组和对照组的无菌盆栽实验,以及主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)、变量投影重要性(VIP)和t检验等统计学方法,筛选出差异代谢物。PCA得分图显示高浓度暴露组(5、10 mg·L^(-1)和20 mg·L^(-1))和低浓度暴露组(0.1 mg·L^(-1)和1 mg·L^(-1))呈现出显著的分离。低暴露组发现了11~24种差异代谢物,而高暴露组发现了23~29种差异代谢物。低暴露组中受到干扰的代谢通路为氧化应激反应中的苯丙烷代谢和能量代谢中的糖代谢,而高暴露组受到干扰的代谢通路为氨基酸代谢、不饱和脂肪酸的生物合成、氨酰tRNA生物合成、苯丙烷生物合成、角质、木栓碱和蜡质的生物合成、脂肪酸生物合成、芥子油甘生物合成和α-亚麻酸代谢,以及硫代谢等多种复杂代谢通路。研究发现高、低浓度PFOS对拟南芥叶片造成了不同的代谢毒性,拟南芥对不同浓度的PFOS启动了不同的代谢防御机制。
Information regarding the toxic effects of high concentration of perfluorooctane sulfonates(PFOS)on plants cannot be used as the standard for determining the toxic effects of low concentration of PFOS on the environment.Moreover,the various toxic effects of different levels of PFOS are still not clear.To better understand the toxic effects of different concentrations of PFOS on plants,a highthroughput non-targeted metabolomics technique was used to study the metabolic effects of different concentrations of PFOS on Arabidopsis thaliana.Several differential metabolites were screened through sterile pot experiments using exposure and control groups.Data were analyzed statistically using methods,such as PCA,OPLS-DA,VIP,and t-test.PCA analysis showed significant separation between the high and low exposure groups.11~24 biomarkers were found in the low-exposure groups(0.1 mg·L^(-1)and 1 mg·L^(-1)),whereas 23~29biomarkers were found in the high-exposure groups(5,10 mg·L^(-1),and 20 mg·L^(-1)).The disturbed metabolic pathways in the low exposure group were phenylpropanoid biosynthesis in oxidative stress and sugar metabolism in energy metabolism,whereas the disturbed metabolic pathways in the high exposure group were amino acid metabolism;unsaturated fatty acid,aminoacyl tRNA,phenylpropanoid,cutin,suberine,wax,fatty acid,and canola glycerol biosynthesis;andα-linolenic acid and sulfur metabolism.This study revealed that high and low levels of PFOS caused different metabolic toxicity in Arabidopsis thaliana leaves,and Arabidopsis thaliana initiated different metabolic defense mechanisms against different concentrations of PFOS.The results provide theoretical basis for studying the toxic effects of different levels of perfluorinated compounds on the environment.
作者
张越
刘潇威
张艳伟
张景然
刘冰洁
ZHANG Yue;LIU Xiaowei;ZHANG Yanwei;ZHANG Jingran;LIU Bingjie(Agro-Environmental Protection Institute,Ministry of Agriculture and Rural Affairs,Tianjin 300191,China;Shanghai SCIEX Analytical Instrument Trading Co.,Ltd.,Shanghai 200335,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2022年第9期1897-1907,共11页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(41807391)。
