摘要
目的:建立一测多评(QAMS)法同时测定连花清瘟胶囊中新绿原酸、绿原酸、隐绿原酸、异连翘酯苷A、槲皮苷、4,5-二-O-咖啡酰奎宁酸和甘草酸7个成分的含量。方法:采用Waters Acquity UPLC H-Class超高效液相色谱系统(PDA检测器),ACQUITY UPLC^(■)HSS T3色谱柱(100 mm×2.1 mm, 1.8μm),以甲醇(A)-乙腈(B)-0.1%磷酸水(C)为流动相,梯度洗脱,流速0.3 mL^(-1)·min^(-1)(0~8 min^(-1))、0.4 mL^(-1)·min^(-1)(9~60 min^(-1)),柱温35℃,检测波长为327 nm(新绿原酸、绿原酸、隐绿原酸、异连翘酯苷A和4,5-二-O-咖啡酰奎宁酸)和250 nm(槲皮苷和甘草酸)。结果:新绿原酸、绿原酸、隐绿原酸、异连翘酯苷A、槲皮苷、4,5-二-O-咖啡酰奎宁酸、甘草酸的线性范围分别为1.54~61.72、3.88~155.16、1.68~67.32、4.78~191.04、1.13~45.32、1.49~59.52、2.10~84.10μg·mL^(-1)(r≥0.999 5);平均回收率(n=9)为98.3%~101.2%,精密度、重复性、稳定性RSD均≤3%;QAMS法测得12批样品中新绿原酸、绿原酸、隐绿原酸、异连翘酯苷A、槲皮苷、4,5-二-O-咖啡酰奎宁酸和甘草酸的含量范围分别为1.348~2.004、1.929~2.442、1.118~1.881、3.512~6.689、0.393~0.594、0.524~0.846、1.735~1.945 mg·g,QAMS法计算值与ESM实测值间均无显著性差异。结论:本法同时测定连花清瘟胶囊中7个化合物含量准确可靠,可用于连花清瘟胶囊质量控制。
Objective:To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determin^(-1)ation of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A, quercitroside, 4,5-di-O-caffeoylquinic acid and glycyrrhizic acid in Lianhua Qingwen capsules. Methods: Analysis was performed on a Waters Acquity UPLC H-Class with PDA detector and anACQUITY UPLC^(■) HSS T3 column(100 mm×2.1 mm, 1.8 μm). The mobile phase consisted of methanol-acetonitrile-0.1% phosphoric acid aqueous in gradient mode. The flow rate was 0.3 mL^(-1)·min^(-1)(0-8 min^(-1)) and 0.4 mL^(-1)·min^(-1)(9-60 min^(-1)), the column temperature was maintained at 35 ℃ and the detection wavelengths were 205 nm(quercitroside and glycyrrhizic acid) and 327 nm(neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A and 4,5-di-O-caffeoylquinic acid). Results: neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A, quercitroside, 4,5-di-O-caffeoylquinic acid and glycyrrhizic acid showed good linear relationship within the ranges of 1.54-61.72, 3.88-155.16, 1.68-67.32, 4.78-191.04, 1.13-45.32, 1.49-59.52 and 2.10-84.10 μg·mL^(-1), respectively(r≥0.999 5). The average recoveries of the seven compounds were within 98.3%-101.2%. Results of precision, stability and reproducibility test were satisfactory. The contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoforsythiaside A, quercitroside, 4,5-di-O-caffeoylquinic acid and glycyrrhizic acid in twelve batches of samples were 1.348-2.004, 1.929-2.442, 1.118-1.881, 3.512-6.689, 0.393-0.594, 0.524-0.846 and 1.735-1.945 mg·gby QAMS. There was no significant difference between the QAMS and ESM. Conclusion: The established QAMS can quantify eight components in Lianhua Qingwen capsules accurately and reliably. This developed method can be used to determin^(-1)e multiple components in Lianhua Qingwen capsules.
作者
孙云波
陈育鹏
张创峰
薛磊
陈晨
任文学
SUN Yun-bo;CHEN Yu-peng;ZHANG Chang-feng;XUE-Lei;CHEN Chen;REN Wen-xue(Beijing Yiling Pharmaceutical Co.,Lid.,Bejing 102600,China;Hebei Province Institute of Integrated Traditional and Westem Medicine,Shijazhuang 050035.,China;Shijazhuang Yiling Pharmaceutial Co.,Lud.,Shjiazhuang 050035,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2022年第7期1128-1136,共9页
Chinese Journal of Pharmaceutical Analysis
关键词
连花清瘟胶囊
新绿原酸
绿原酸
隐绿原酸
异连翘酯苷A
槲皮苷
4
5-二-O-咖啡酰奎宁酸
甘草酸
一测多评(QAMS)
超高效液相色谱
Lianhua Qingwen capsules
neochlorogenic acid
chlorogenic acid
cryptochlorogenic acid
isoforsythiaside A
quercitroside
4,5-di-O-caffeoylquinic acid
glycyrrhizic acid
quantitative analysis of multi-components by single-marker(QAMS)
UPLC
