摘要
目的 对杜仲TCP(teosinte branched 1/cycloidea/proliferating)基因家族进行筛选分析,以期为EuTCPs基因功能深入研究奠定基础。方法 以杜仲基因组数据库为基础,通过生物信息学系统分析TCP基因家族的理化性质、系统进化、基因结构、启动子顺式作用元件及其在杜仲叶片发育及胶形成中的表达水平。结果 从杜仲基因组中共鉴定到14个EuTCPs,氨基酸数目介于139~492,理论等电点分布在5.54~9.72,相对分子质量区域为18 880~53 620,亚细胞定位预测在细胞核中,均为亲水性蛋白。系统进化分为PCF、CIN和CYC/TB13个亚家族,分别包含5、6、3个EuTCPs蛋白。表达模式分析显示,EuTCPs在杜仲叶片不同发育时期存在显著差异,每个发育时期EuTCPs表达量各不相同,EuTCPs正调控杜仲胶的形成。结论 杜仲中TCP家族包含14个成员,各成员的分子特征和组织表达特异性存在差异,EuTCPs可能参与杜仲叶片发育及杜仲胶的形成。
Objective To screen and analyze TCP(Teosinte branched 1/Cycloidea/Proliferating) gene family of Eucommia ulmoides, so as to lay a foundation for further study of EuTCPs gene function research. Methods Based on E. ulmoides genome database, the physicochemical properties, phylogeny, gene structure, promoter cis-acting elements and their expression levels in the leaf development and eu-rubber formation of the TCP gene family were analyzed by bioinformatics system. Results A total of 14EuTCPs were identified from E. ulmoides genome, EuTCPs encoded 139—492 amino acids, and theoretical isoelectric points distribution were 5.54—9.72, molecular weight was between 18 880 to 53 620 by physicochemical analysis, all of EuTCPs proteins were hydrophilic proteins and expressed in nucleus. EuTCP gene family was divided into three subfamilies: PCF, CIN and CYC/TB1, which contained five, six and three EuTCPs proteins, respectively. Expression pattern analysis showed that EuTCPs were significantly different at different developmental stages of E. ulmoides leaves, and the expression levels of EuTCPs were different at each developmental stage. EuTCPs positively regulated the formation of eu-rubber. Conclusion There are 14 members of TCP family identified in E. ulmoides, and their molecular characteristics and tissue expression specificity are different, which may be involved in the development of leaves and eu-rubber formation.
作者
刘俊
李龙
吴耀松
刘燕
任闪闪
陈玉龙
LIU Jun;LI Long;WU Yao-song;LIU Yan;REN Shan-shan;CHEN Yu-long(Henan key Laboratory of TCM Prescription and Syndrome Signaling,Academy of Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China;College of Forestry,Northwest Agriculture and Forestry University,Yangling 712100,China;International Center for Bamboo and Rattan,Key Open Laboratory of Bamboo and Rattan Science and Technology,China National Forestry and Grassland Administration,Beijing 100102,China;Anhui Taiping Experimental Station of International Center for Bamboo and Rattan,Huangshan 245716,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第18期5813-5824,共12页
Chinese Traditional and Herbal Drugs
基金
河南省高等学校重点科研项目(22A360005)
国家林业和草原局/北京市共建竹藤科学与技术重点实验室开放基金(ICBR-2020-05)
国际竹藤中心安徽太平试验中心开放课题基金(1632021006-4)
河南中医药大学博士科研基金资助项目(RSBSJJ2019-04)。
关键词
杜仲
TCP转录因子
亚细胞定位
系统进化
表达分析
Eucommia ulmoides Oliver
TCP gene family
subcellular location
phylogeny evolution
expression analysis
