摘要
目的 探讨不同产地和生长年限对巴戟天Morinda officinalis中环烯醚萜苷类成分形成和积累的影响。方法 以HPLC法分析巴戟天环烯醚萜苷类成分的含量。色谱柱为艾杰尔Venusil MP C_(18)色谱柱(250 mm×4.6 mm,5μm);以0.2%磷酸+0.01 mol/L磷酸氢二钠缓冲盐(A)-乙腈(B)为流动相,梯度洗脱;体积流量1.0 mL/min,柱温25℃;二极管阵列检测器(DAD)检测。结果 巴戟天中水晶兰苷、去乙酰基车叶草苷酸、车叶草苷酸和车叶草苷4个成分在各自质量浓度范围内线性关系良好,精密度、重复性、稳定性和加样回收率均符合含量测定要求。巴戟天环烯醚萜苷类成分广西产质量分数为(17.74±0.49)~(60.67±0.28)mg/g,广东产质量分数为(28.46±0.68)~(58.29±0.38)mg/g,福建产质量分数为(20.75±0.42)~(43.94±1.10)mg/g。总量以7年生广西梧州产最高,为(60.67±0.28)mg/g,其次是3年生广东高要产,为(58.29±0.38)mg/g。结论 通过23批不同产地及生长年限的巴戟天药材环烯醚萜苷类成分含量对比,发现7年生广西梧州产巴戟天环烯醚萜苷类成分含量最高,3年生广东高要次之,为巴戟天药材合理的采收时间和质量控制提供参考依据。
Objective To explore the effects of geographical location and growth period on the formation and accumulation of iridoid glycosides in Morinda officinalis. Methods HPLC was used to analyze the content of iridoid glycosides. The chromatography conditions were as follows: HPLC column was Venusil MP C_(18)(250 mm × 4.6 mm, 5 μm). The mobile phase was 0.2% phosphoric acid water + disodium hydrogen phosphate buffered salt(A)-acetonitrile(B), and gradient elution with the flow rate of 1.0 mL/min. The column temperature was 25 ℃ and detector was diode array detector(DAD). Results The four components(monotropein, deacetyl asperuloside acid, asperuloside asperuloside, asperuloside) all showed good linear relationship in their respective ranges, precisions, repeatability, stability and recoveries conformed the requirements of content determination. The contents of MOIG from Guangxi ranged from(17.74 ± 0.49) to(60.67 ± 0.28) mg/g, Guangdong ranged from(28.46 ± 0.68) to(58.29 ± 0.38) mg/g, Fujian ranged from(20.75 ± 0.42) to(43.94 ± 1.10) mg/g. Among them, the total MOIG content of 7-year-old Wuzhou was the highest at(60.67 ± 0.28) mg/g. Secondly, the content of 3-year-old Gaoyao was(58.29 ± 0.38) mg/g. Conclusion By comparing the content of MOIG in 23 batches of different geographical location and growth period, it was found that the MOIG content of 7-year-old Wuzhou was the highest, followed by the 3-year-old Gaoyao. This research may provide reference for the reasonable harvest time and the quality control of M. officinalis.
作者
沈燚
孙艺琦
张奇
李鹤鸣
赵子慧
刘沙
张泉龙
秦路平
赵瑛
张巧艳
SHEN Yi;SUN Yi-qi;ZHANG Qi;LI He-ming;ZHAO Zi-hui;LIU Sha;ZHANG Quan-long;QIN Lu-ping;ZHAO Ying;ZHANG Qiao-yan(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China;Xianyang Central Hospital,Xianyang 712000,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第17期5484-5490,共7页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(U1505226)
浙江省自然科学基金重点项目(LZ22H28002)。
