摘要
【目的】研究环丙氨嗪对鸡粪中亮斑扁角水虻Hermetia illucens幼虫生长发育的影响及添加活性炭对环丙氨嗪的解毒作用。【方法】使用含不同浓度(0,0.1,0.25,0.5,1,2.5,5,10和15 mg/kg)环丙氨嗪的鸡粪分别饲养3,4和5龄亮斑扁角水虻幼虫,统计幼虫的生长发育(体重和死亡率)变化;在鸡粪中添加不同浓度(0,10,30,50,100和150 g/kg)活性炭饲养亮斑扁角水虻3龄幼虫,测定活性炭对其生长发育的影响以及对环丙氨嗪的解毒作用。【结果】在用含一定浓度环丙氨嗪的鸡粪饲养后,亮斑扁角水虻3,4和5龄幼虫均表现出体节伸长,停止进食,直至死亡。鸡粪中环丙氨嗪对亮斑扁角水虻3,4和5龄幼虫的致死中浓度(medium lethal concentration,LC_(50))值分别为0.18,1.39和6.45 mg/kg。在鸡粪中添加量为30~100 g/kg的活性炭可以显著促进亮斑扁角水虻3龄幼虫的生长发育,并且能够解除环丙氨嗪对3龄幼虫的毒性,95%有效剂量(95%effective dose,ED_(95))值为51.83 g/kg。【结论】随着亮斑扁角水虻幼虫龄期增长,其对鸡粪中环丙氨嗪的敏感性逐渐降低。活性炭可用于解除鸡粪中环丙氨嗪对亮斑扁角水虻幼虫的毒性,为生产上应用亮斑扁角水虻处理含环丙氨嗪的鸡粪提供解决方案。
【Aim】This study aims to study the effects of cyromazine on the growth and development of the black soldier fly(Hermetia illucens)larvae in chicken manure and the detoxification of cyromazine by the added activated carbon.【Methods】The 3rd,4th and 5th instar larvae of H.illucens were reared in the chicken manure containing different concentrations(0,0.1,0.25,0.5,1,2.5,5,10 and 15 mg/kg)of cyromazine,and the changes in the growth and development(body weight and mortality)of the larvae were measured.Reared in the chicken manure added with activated carbon at different concentrations(0,10,30,50,100,and 150 g/kg),the effects of activated carbon on the growth and development of the 3rd instar larvae of H.illucens and its detoxification effect on cyromazine were assayed.【Results】Reared in the chicken manure containing certain concentration of cyromazine,the 3rd,4th and 5th instar larvae of H.illucens showed such symptoms as elongation of somites,cessation of feeding and even death.The medium lethal concentration(LC_(50))values of cyromazine to the 3rd,4th and 5th instar larvae of H.illucens were 0.18,1.39 and 6.45 mg/kg,respectively.Activated carbon at the concentrations of 30-100 g/kg in chicken manure significantly promoted the growth and development of the 3rd instar larvae of H.illucens and detoxified the toxicity of cyromazine to the 3rd instar larvae with the 95%effective dose(ED_(95))value of 51.83 g/kg.【Conclusion】The sensitivity of H.illucens larvae to cyromazine in chicken manure gradually decreases with larval instar.Activated carbon can be used to detoxify the toxicity of cyromazine in chicken manure to H.illucens larvae,providing a solution for application of H.illucens to process chicken manure containing cyromazine.
作者
张海旭
裴亚欣
张继冉
索江华
邓新
陈红歌
林晖
杨森
ZHANG Hai-Xu;PEI Ya-Xin;ZHANG Ji-Ran;SUO Jiang-Hua;DENG Xin;CHEN Hong-Ge;LIN Hui;YANG Sen(College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2022年第9期1166-1176,共11页
Acta Entomologica Sinica
基金
国家重点研发计划(2021YFD1700900)
国家自然科学基金项目(31801672)
河南省青年人才托举工程资助项目(2022HYTP041)。
关键词
亮斑扁角水虻
环丙氨嗪
活性炭
鸡粪
解毒
Hermetia illucens
cyromazine
activated carbon
chicken manure
detoxification
