摘要
我们在进行毛冠鹿的染色体研究时,观察到不仅该动物染色体核型特殊,而且结构异染色质丰富,尤其是23号、24号、25号染色体几乎占长臂的3/4甚至全部(张钖然等,1983),这在其他哺乳动物的染色体中尚少见,建立该动物之细胞株,对进一步探讨鹿科动物的染色体进化,对研究结构异染色质的结构和功能,提供了一个较好的材料。本文报道了毛冠鹿细胞株的建立,命名为KIZ—81A,并对其部分生物学特性进行了观察研究。
In this paper we present a cell strain (designated KIZ-81A) derived from lung tissue of a young male tufted deer (Elaphodus cephalophus).The animal was caught in southern China in 1981.The results obtained from our observations on the biological characteristics of KZI-81A strain are as follows.1.The KIZ-8lA cells were fibroblasts in morphology.The primary cells died at passage 67 after 13 months in vitro,while KIZ-8lA cells,which had been frozen since passage 3 and kept in liquid nitrogen for 132 days before thawing,attained only 64 passages.2.Growth rates of KIZ-81A cells were determined at passage 7,11,30.Comparison was made at generation 11 of primary cells and cells once frozen.The results showed that after a lag phase,all cultures entered the logarithmic growth phase and then the stationary phase again.3.The range of cell cycle time,determined by BUdR-Gicmsa method for detection of sister chromatid exchanges,was from 24 to 36 h.4.Karyotypc analysis revealed the chromosome number and construction in KIZ-8lA cells (without being frozen and stored) were stabler than those of the cells once frozen in liquid nitrogen.In the former,the obvious increase of chromosome number and changes in construction started from the 51th generation and in the latter from the 30th generation.The cells with chromosome number from 49 to 52 greatly increased.The effects of some experimental factors,including temperature and pH changes,on cell growth and division arc also analysed and discussed.
作者
张锡然
王建华
陈玉泽
Zhang Xiran;Wang jianhua;Chen Yuze(Kunming Institute of Zoology, Academia Sinica)
