摘要
本文用骨髓及精巢作实验材料,采用秋水仙素—低渗—空气干燥直接制片法,首次报道了昆明龙蜥Japalura vacoae(Boulenger)的核型,即2n=12+22mNF=46,并与已经报道的本属的另二个物种—J.swinhonis J.polygonata—的核型作了比较。讨论了它们的核型演化途经。在该属染色体臂数具有明显的保守性。因此,罗伯逊易位在演化中起着十分重要的作用。结合核型分析还讨论了龙蜥属的分布和起源中心。另外,本文还观察分析了精巢的减数分裂。昆明龙蜥—Japalura varcoae(Boulenger)—属蜥蜴目(Lacertiformes),鬣蜥科(Agamidae),分布在我国的云、贵高原(川生所,1977),是高原山地型种类。各种动物的核型研究,不但对于细胞学、遗传学是项基本工作,同时还可以丰富动物的信息系统,为动物演化的研究提供宝贵资料。据查,昆明龙蜥的核型未曾报道。特作此文。
In this study,the karyotype of Japalura varcoae had been firstly reported.The examined materials are metaphascs of the mitosis and meiosis from marrow cells and test tissue,which prepared by colchicine-hypotonic-air drying technique.The karyotype is 2n=12V+22m(V is used for metacentric chromosome of the macrochromosome,m for microchromosome),NF=46(v have a value of 2,all microchromosomes,regardless of morphology,have a value of 1).The comparison with J.swinhonis and J.polygonata was made.The evolutinary process of karyotypes is discussed.It seems that,in this genus,the number of arms in the karyotype is very conservative.Hence the Present adress:Department of Biology,Yunnan University.Robertsonian translocation,accompanied by cenlric fusion,may play important role.In the later,the distributed and original center for this generic Lizards have been discussed with the karyotypical data.
作者
李树深
王应祥
王蕊芳
李崇云
刘光佐
Li Shu-shen;Wang Ying-xiang;Wang Rui-fang;Li Chong-yun;Liu Guang-zuo(Kunming Institute of Zoology,Academia Sinica)
