摘要
本研究收集了19株不同来源的斑玉蕈品种,通过ITS测序构建其系统发育树,随后根据菌株在木屑培养基与葡萄糖培养基的生长速度对菌株进行分类,选取生长速度差异明显的快、中和慢8株斑玉蕈菌株进行出菇实验及胞内外碳代谢指标的测定,探讨斑玉蕈生产性能与胞内外碳代谢的相关性。研究发现收集到的19株菌株均为斑玉蕈,亲缘关系近,遗传分化程度低;选取出的8株斑玉蕈菌株鲜菇产量与菌丝生长速度呈极显著正相关,菌丝生长越快,出菇产量越高。同时,斑玉蕈菌株鲜菇产量与菌丝湿重、还原糖、可溶性蛋白、滤纸酶(FPase)、CMC-Na酶(CMCase)、木聚糖酶和淀粉酶7个胞外碳代谢指标(ECMI)及胞内葡萄糖、己糖激酶(HK)、丙酮酸激酶(Pyk)、柠檬酸合酶(CS)、α-酮戊二酸脱氢酶(KGDH)和葡萄糖-6-磷酸脱氢酶(G6PD)6个胞内碳代谢指标(ICMI)呈显著正相关。说明菌丝生长速度快的斑玉蕈品种,胞外基质分解速度更快,提高可吸收碳源的供应,胞内加强对碳的吸收与同化,为菌丝的增殖提供更多原料与能量。本研究分析了斑玉蕈生产性能与胞内外碳代谢的相关性,为斑玉蕈优良品种的鉴别与选育奠定基础。
In this study,19 Hypsizygus marmoreus strains were collected from different sources and identified via ITS sequencing.Of which 8 strains cultured on sawdust medium and glucose medium with difference of growth rate(fast,intermediate and slow growing)were selected for performing fruiting experiment and intracellular and extracellular carbon metabolism assays to explore the correlation between fruiting yield and carbon metabolism.The ITS sequencing indicated that 19 collected strains were genetically closely relative,with low genetic differentiation.The 8 selected strains appeared significantly positive correlation between mycelial growth rate and fruiting yield,showing the faster mycelium grew,the higher fruiting yield.Furthermore,seven extracellular carbon metabolic indexes(ECMI)including wet mycelial weight,reducing sugar,soluble protein,enzyme activity of FPase,CMCase,xylanase,amylase,and six intracellular carbon metabolic indexes(ICMI)including intracellular glucose,HK,Pyk,CS,KGDH and G6PD also showed significantly positive correlation with the fruiting yield,demonstrating that faster growth rate of mycelium could accelerate the decomposition of substrate to improve the supply of available carbon source through secreting more extracellular enzymes,and strengthen intracellular carbon assimilation to generate more energy for mycelium proliferation and fruiting body formation.This study provides technical and theoretical reference for breeding and identification of superior H.marmoreus varieties.
作者
林辉
李娜娜
李佳欢
孙淑静
张燎原
胡开辉
LIN Hui;LI Nana;LI Jiahuan;SUN Shujing;ZHANG Liaoyuan;HU Kaihui(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Edible Fungal Research Institute(Gutian)of Fujian Agriculture and Forestry University,Ningde 352200,Fujian,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2022年第9期1506-1518,共13页
Mycosystema
基金
国家自然科学基金面上项目(31870481)
福建省自然科学基金重点项目(2019J02007)
2020–2022福建省现代食用菌产业技术体系建设(闽农综[144]号)。
关键词
斑玉蕈
生产性能
胞内碳代谢指标
胞外碳代谢指标
相关性分析
Hypsizygus marmoreus
production performance
intracellular carbon metabolism indexes
extracellular carbon metabolism indexes
correlation analysis
