摘要
目的系统评价以半夏泻心汤为基础方治疗复发性口腔溃疡(recurrent oral ulcer,ROU)的有效性及复发率。运用网络药理学方法初步研究半夏泻心汤治疗ROU的作用机制。方法检索中国知网、万方数据库、中国中医药数据库、维普资讯中文期刊服务平台、PubMed数据库,纳入相关的临床对照研究,检索时间期限为建库至2021年12月30日,根据纳入和排除标准确定最终纳入文献,对纳入文献进行方法学质量评价,运用Review Manager 5.3软件进行Meta分析。运用TCMSP数据库获取半夏泻心汤有效成分及预测药物作用靶点,运用GeneCards和OMIM数据库获取ROU疾病基因,使用R软件获得交集靶点,使用Cytoscape 3.9.0软件绘制疾病-中药-靶点网络图。运用STRING在线工具和Cytoscape 3.9.0软件构建处理蛋白互作PPI网络,使用Cytoscape 3.9.0软件的ClueGo插件进行GO富集及KEGG通路分析。结果最终纳入13篇文献,Meta分析结果显示以半夏泻心汤为基础方治疗ROU总有效率[OR=6.62,95%CI(4.40,9.96),P<0.00001]优于对照组;3个月复发率[OR=0.25,95%CI(0.10,0.63),P=0.003]、6个月复发率[OR=0.27,95%CI(0.13,0.59),P=0.0009]均小于对照组,差异均具有统计学意义。半夏泻心汤与ROU的交集靶点共201个,GO富集分析显示功能涉及细胞凋亡过程、细胞内信号转导、蛋白质磷酸化、细胞迁移、程序性细胞死亡等,KEGG分析显示其与癌症通路、感染相关通路、AGE-RAGE信号通路、PI3K-Akt信号通路、MAPK信号通路、TNF信号通路、IL-17信号通路等有关。结论相对于对照组,以半夏泻心汤为基础方治疗ROU疗效更好,复发率更低。半夏泻心汤治疗ROU是多靶点、多通路的复杂过程,其机制研究还需更多的实验数据支撑。
Objective To evaluate the effectiveness and recurrence rate of Banxiaxiexin decoction in the treatment of recurrent oral ulcer(ROU).The mechanism of Banxiaxiexin decoction on ROU was studied by network pharmacology methods.Methods According to the retrieval rules of CNKI,Wan-Fang Data,TCMDB,VIP and PubMed databases,the corresponding search terms were formulated to retrieve the relevant literature,screened the literature consistent with the standards,and then Review Manager5.3 statistical software was used for Meta-analysis of the selected literature.TCMSP database was used to obtain the effective components of Banxiaxiexin decoction and predict drug targets.GeneCards and OMIM database were used to obtain ROU disease genes.R software was used to obtain intersection targets.Cytoscape 3.9.0 software was used to draw the disease-Traditional Chinese Medicine-target network diagram.The protein interaction network was constructed by using STRING online tool and Cytoscape 3.9.0 software,and GO enrichment and KEGG pathway analysis were performed by using ClueGo plug-in of Cytoscape 3.9.0 software.Results Thirteen literature were included,and the results of Meta-analysis showed that the total effective rate of Banxiaxiexin decoction in the treatment of ROU[OR=6.62,95%CI(4.40,9.96),P<0.00001],which was significantly better than the control group.Recurrence rate in three months[OR=0.25,95%CI(0.10,0.63),P=0.003],recurrence rate in six months[OR=0.27,95%CI(0.13,0.59),P=0.0009]was markedly lower than that of the control group.There were 201 overlapping genes between the target of Banxiaxiexin decoction and ROU,whose functions involved apoptosis,intracellular signal transduction,protein phosphorylation,cell migration,programmed cell death in GO analysis.These overlapping genes were related to cancer pathway,infection-related pathway,AGE-RAGE signaling pathway,PI3K-Akt signaling pathway,MAPK signaling pathway,TNF signaling pathway,IL-17 signaling pathway in KEGG analysis.Conclusion Compared with the control group,the treatment of ROU based on Banxiaxiexin decoction has better efficacy and lower recurrence rate.Banxiaxiexin decoction treatment for ROU is a multi-target,multi-pathway complex process,and its mechanism needs more experimental data support.
作者
韩春雯
张丽娜
汪梅姣
张祎
高燕
李海昌
谢志军
HAN Chunwen;ZHANG Lina;WANG Meijiao;ZHANG Yi;GAO Yan;LI Haichang;XIE Zhijun(Zhangzhou Health Vocational College,Zhangzhou 363000,China;School of Basic Medical Sciences,Zhejiang Chinese Medicine University,Hangzhou 310053,China)
出处
《云南中医学院学报》
2022年第2期75-82,共8页
Journal of Yunnan University of Traditional Chinese Medicine
基金
福建省卫生计生青年科研课题(2018-2-46)
福建省中青年教师教育科研项目(JZ180851)
浙江省中医药科技计划重点研究项目(2020ZZ006)。
