摘要
目的探究山豆根对金黄色葡萄球菌(以下简称为金葡菌)毒力表达的影响,为临床用药进一步提供实验数据,丰富山豆根抗菌药用价值参考资料。方法分别采用紫外吸光度法、扫描电镜、溶血试验法和Western Blot检测了山豆根作用后金葡菌细胞膜通透性、菌体形态变化,溶血活性和外毒素的表达。结果亚抑菌浓度的山豆根能破坏细胞膜完整性并引起菌体变化,降低溶血能力和α-溶血素、金葡菌A型肠毒素(SEA)和金葡菌B型肠毒素(SEB)分泌。结论山豆根对金葡菌毒力有抑制作用。
Objective To explore the effect of Sophora tonkinensis Gagnep on the expression of virulence of Staphylococcus aureus,and to provide further experimental data for clinical drug use,so as to enrich the reference materials of medicinal antimicrobial value of Sophora tonkinensis Gagnep.Methods Ultraviolet absorbance,scanning electron microscopy,hemolysis assay and Western blot were used to detect the membrane permeability,morphological changes,hemolytic activity and exotoxin expression of Staphylococcus aureus.Results The integrity of cell membrane,hemolytic ability and secretion ofα-hemolysin,SEA and SEB were damaged at subinhibitory concentration.Conclusion Sophora tonkinensis Gagnep can inhibit the expression of Staphylococcus aureus virulence.
作者
黄少隆
张翠惠
黄冬枚
郭文婷
郑庆茹
周湧
HUANG Shaolong;ZHANG Cuihui;HUANG Dongmei;GUO Wenting;ZHENG Qingru;ZHOU Yong(Department of Clinical Laboratory,Binhaiwan Central Hospital of Dongguan,Dongguang,Guangdong 523903,China)
出处
《检验医学与临床》
CAS
2022年第20期2803-2807,共5页
Laboratory Medicine and Clinic
基金
广东省东莞市科学技术局项目(202050715025009)。
关键词
山豆根
金黄色葡萄球菌
毒力表达
Sophora tonkinensis Gagnep
Staphylococcus aureus
virulence express
