摘要
为了精准和快速检测副流感病毒5型(PIV5),本研究下载NCBI中发布的不同物种来源的25株PIV5全基因序列,对比后选择高度保守的NP基因序列区段设计特异性引物,建立了检测PIV5的RT-PCR方法。结果显示,建立的方法具有较强的敏感性和特异性,可扩增出115 bp大小的片段。敏感性结果显示,核酸最低检测量为7.93×10^(5)copies/μL,与牛副流感3型病毒等8种临床常见病毒均无交叉反应。分别采集猪、牛及犬的临床样品共40份进行检测,阳性率为20%,表明建立的RT-PCR方法可用于临床上副流感病毒的检测。
In order to accurately and quickly detect parainfluenza virus type 5(PIV5),this study downloaded the whole gene sequences of 25 strains of PIV5 from different species published in NCBI,aligned and selected highly conserved NP gene sequence segments to design specific primers,and established RT-PCR method for detecting PIV5.The results showed that the established method had strong sensitivity and specificity,and the lowest detection of nucleic acid was 7.93×10^(5)copies/μL.There was no cross reaction with 8 common clinical viruses such as bovine parainfluenza 3 virus.A total of 40 clinical samples from pigs,cattle and dogs were detected by this method,and the positive rate was 20%,indicating that the established RT-PCR method can be used for the detection of parainfluenza virus in clinic.
作者
王岩
谭斌
刘可欣
王倩颖
杨森
张淑琴
WANG Yan;TAN Bin;LIU Ke-xin;WANG Qian-ying;YANG Sen;ZHANG Shu-qin(Key Laboratory of Economic Animal Diseases,The Ministry of Agriculture/Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130122)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2022年第9期1108-1114,共7页
Chinese Veterinary Science
基金
吉林省创新创业人才项目(2021Y034)
吉林省重点研发项目(20210202044NC)。
