摘要
采用醇碱溶、酸沉析的方法对青稞酒糟蛋白质进行提取。在单因素试验的基础上,选取浸提液醇碱比、固液比、浸提时间和浸提温度为影响因子,采用4因素3水平的正交试验,以蛋白质提取率作为指标,确定最佳提取工艺条件为浸提液醇碱比1∶3,固液比1∶45,浸提温度30℃,浸提时间90 min,各因素对蛋白质提取率的影响强弱顺序为固液比>醇碱比>浸提温度>浸提时间。
The protein of highland barley distiller’s grains was extracted by alcohol-alkali dissolution and acid precipitation.Based on the single factor experiment,the alcohol-alkali ratio,solid-liquid ratio,extraction time and extraction temperature were selected as the influencing factors.Using the orthogonal experiment of four factors and three levels,and taking the protein extraction rate as the in⁃dex,the optimal extraction process condition was alcohol-alkali ratio of 1∶3,solid-liquid ratio of 1∶45,extraction temperature of 30℃,and extraction time of 90 min.The order of the influence of the various factors on the protein extraction rate was solid-liquid ra⁃tio>alcohol-alkali ratio>extraction temperature>extraction time.
作者
樊世萌
刘浩
欧阳敬禹
刘恋
尤祥宇
苏江涛
肖红卫
FAN Shi-meng;LIU Hao;OUYANG Jing-yu;LIU Lian;YOU Xiang-yu;SU Jiang-tao;XIAO Hong-wei(School of Biological Engineering and Food,Hubei University of Technology,Wuhan 430068,China;Xiangyang Industrial Institute of Hubei University of Technology,Xiangyang 441100,Hubei,China;Hubei Provincial Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430072,China)
出处
《湖北农业科学》
2022年第18期169-172,共4页
Hubei Agricultural Sciences
基金
动物胚胎工程及分子育种湖北省重点实验室开放课题(KLAEMB-2019-02)
襄阳湖北工业大学产业研究院2022年度项目(XYYJ2022C13)。
关键词
青稞酒糟
蛋白质提取
正交试验
highland barley distiller’s grains
protein extraction
orthogonal test
