摘要
以酰肼功能团修饰的胶体金作为试纸条的标记材料,利用酰肼基团可特异性地与抗体Fc片段醛基发生亲核加成反应的特性,实现抗体在胶体金纳米粒子(gold nanoparticles,AuNPs)表面的定向偶联。对比于碳二亚胺介导的共价偶联法,酰肼功能团介导的定向偶联需要更少的抗体标记量,更短的偶联时间,酰肼化胶体金免疫层析试纸条(hydrazided gold nanoparticles-based immunochromatographic assay,hAuNPs-ICA)检测玉米赤霉烯酮(zearalenone,ZEN)展示出更好的检测灵敏度;将hAuNPs-ICA检测ZEN加标的玉米样品,结果显示加标回收率介于83.1%~118.0%,批内、批间变异系数为3.9%~13.1%,表明hAuNPs-ICA具有较好的精密度和准确性。
Anti-zearalenone monoclonal antibodies(mAbs) were directionally conjugated on the surface of hydrazide group-functionalized gold nanoparticles because of the nucleophilic addition reaction between the aldehyde groups of the antibody Fc region and the hydrazide groups on gold nanoparticles(AuNPs). Compared with the carbodiimide-mediated covalent coupling method, the proposed directional coupling strategy showed several advantages such as use of less amounts of labeled antibody and shorter reaction time. The hydrazided gold nanoparticles-based immunochromatographic assay(hAuNPs-ICA) showed higher sensitivity for ZEN detection. Additionally, when hAuNPs-ICA was used to analyze ZENspiked maize samples, the average recoveries ranged from 83.1% to 118.0% with intra-and inter-batch coefficients of variation ranging from 3.9% to 13.1%, indicating good accuracy and precision.
作者
周梦君
周舒
黄小林
李响敏
熊勇华
ZHOU Mengjun;ZHOU Shu;HUANG Xiaolin;LI Xiangmin;XIONG Yonghua(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;College of Food Science and Technology,Nanchang University,Nanchang 330031,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第20期44-52,共9页
Food Science
基金
“十三五”国家重点研发计划重点专项(2018YFC1602202
2018YFC1602203
2018YFC1602505)
国家自然科学基金地区科学基金项目(31760485)
国家自然科学基金青年科学基金项目(31901780)。
