摘要
目的:提高中华鳖背甲胶原蛋白的提取率和产品纯度。方法:以脱钙率与胶原蛋白回收率为评价指标,考察盐酸浓度、脱钙时间、脱钙温度和料液比4个因素对中华鳖背甲脱钙的影响,应用正交试验对鳖甲钙盐的脱除工艺进行优化,并通过SDS-PAGE、紫外吸收光谱、红外吸收光谱、氨基酸分析等技术分析相同提取条件下脱钙与未脱钙的鳖甲胶原蛋白结构的变化。结果:最佳脱钙工艺条件为盐酸浓度0.8 mol/L、脱钙时间2 h、脱钙温度28℃、料液比1∶25(g/mL),该条件下脱钙率和胶原蛋白回收率分别为(67.35±0.92)%和(90.77±1.08)%。鳖甲经盐酸脱钙处理后其单位质量样品中胶原蛋白含量和提取率显著增加(P<0.05),其胶原蛋白结构特征未发生显著变化。结论:盐酸对鳖甲脱钙有较好的脱钙效果,且对其胶原蛋白结构特性无影响。
Objective:To improve the extraction rate and product purity of Chinese soft-shelled turtle(pelodiscussinensis)dorsal collagen.Methods:The decalcification rate and collagen recovery rate were used as evaluation indicators to investigate the effects of hydrochloric acid concentration,decalcification time,decalcification temperature and solid-liquid ratio on the decalcification of the carapace of Chinese soft-shelled turtle.The removal process of calcium salts was optimized,and the structural changes of decalcified and non-decalcified turtle carapace collagen were analyzed by SDS-PAGE,ultraviolet absorption spectroscopy,infrared absorption spectroscopy,amino acid analysis and other techniques under the same extraction conditions.Results:The optimal decalcification process conditions were hydrochloric acid concentration of 0.8 mol/L,decalcification time of 2 h,decalcification temperature of 28℃,and solid-liquid ratio of 1∶25(g/mL).Under these conditions,the decalcification rate and collagen recovery rate were(67.35±0.92)%and(90.77±1.08)%,respectively.The collagen content and extraction rate per unit mass of soft-shelled turtle carapace were significantly increased(P<0.05)after decalcification with hydrochloric acid,but the structural characteristics of collagen did not change significantly.Conclusion:Hydrochloric acid has a good decalcification effect on the decalcification of turtle carapace,and has no effect on the structural properties of its collagen.
作者
赵琼瑜
胡鉴
陈雨欣
李彩燕
宋伟
ZHAO Qiong-yu;HU Jian;CHEN Yu-xin;LI Cai-yan;SONG Wei(College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo,Zhejiang 315100,China)
出处
《食品与机械》
北大核心
2022年第10期151-157,223,共8页
Food and Machinery
基金
浙江省公益性技术应用研究计划项目(编号:LGN19C190006)
浙江省农业(水产)新品种选育重大科技专项(编号:2021C02069-8-3)
宁波市科技创新重大专项(编号:2021Z009)。
关键词
中华鳖
背甲
脱钙工艺
胶原蛋白
结构特性
Chinese soft-shelled turtle
carapace
decalcification process
collagen
structural characteristics
