摘要
肉桂酰辅酶A还原酶(CCR)是木质素生物合成途径中的关键限速酶,本研究从三个不同辣度的辣椒品种‘美国厚肉圆椒’、‘琼海小米椒’、‘海南黄灯笼’基因组中克隆了CCR1基因的启动子,序列长度分别为2075、2102、2082 bp,序列AT含量都占71%,符合真核植物启动子特征。启动子分析软件PlantCARE预测CCR1基因启动子的序列除含有启动子基本核心元件CAAT-box以及TATA-box外,还包括一些激素、光照、生物胁迫及真菌诱导响应等顺式作用元件。瞬时转化烟草可以驱动GUS基因表达,具有启动活性。本研究结果为阐明CCR基因参与辣椒木质素合成的调控机制及启动子转录作用机理研究奠定进一步的理论基础。
Cinnamyl CoA reductase(CCR)is a key rate-limiting enzyme in the lignin biosynthesis pathway.In this study,the promoter of CCR1 gene was cloned from the genomes of three pepper varieties with different pungency degree:Capsicum annuum,Capsicum frutescens and Capsicum chinense.The sequence lengths were 2075,2102 and 2082 bp,and the AT content of the sequences accounted for 71%,which was consistent with the promoter characteristics of eukaryotic plants.The promoter analysis software PlantCARE predicted that the CCR1gene promoter sequence not only contained the basic core elements of the promoter CAAT-box and TATA-box,but also included some cis-acting elements such as hormone,light,biological stress and fungal induced response.Transient transformation of tobacco can drive the expression of GUS gene and has the initiating activity.The results of this study lay a further theoretical foundation for elucidating the regulatory mechanism of CCR gene involved in lignin synthesis in pepper and the mechanism of promoter transcription.
作者
吴丹
张丽平
张威
孙佩霞
朱婕
汪志伟
邓勤
成善汉
Wu Dan;Zhang Liping;Zhang Wei;Sun Peixia;Zhu Jie;Wang Zhiwei;Deng Qin;Cheng Shanhan(Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,Horticulture College of Hainan University,Haikou,570228)
出处
《分子植物育种》
CAS
北大核心
2022年第18期5938-5946,共9页
Molecular Plant Breeding
基金
国家自然科学基金项目(31760578)
海南省自然科学基金高层次人才项目(2019RC030)共同资助。
