摘要
WRKY是一类重要的转录因子,广泛参与调控植物的生长发育和多种应激反应。本研究基于竹叶花椒不同组织(根,芽,皮刺,果)的转录组测序数据,通过BLAST比对获得WRKY基因序列,并对获得的基因进行生物信息学分析;结合竹叶花椒不同组织基因表达数据,分析参与竹叶花椒生长发育相关的WRKY转录因子。从竹叶花椒转录组共鉴定出43个WRKY转录因子,其中包括Ⅰ型基因6个、Ⅱ-a型基因4个、Ⅱ-b型基因7个、Ⅱ-c型基因13个、Ⅱ-d型基因5个、Ⅱ-e型基因4个、Ⅲ型基因4个。ZaWRKY基因表达热图分析显示,ZaWRKY38基因只在芽中表达,ZaWRKY25、ZaWRKY26、ZaWRKY22、ZaWRKY29、ZaWRKY37、ZaWRKY41基因只在根中表达,ZaWRKY15、ZaWRKY30基因只在果中表达,ZaWRKY1、ZaWRKY2、ZaWRKY10、ZaWRKY18、ZaWRKY28、ZaWRKY36、ZaWRKY43基因只在皮刺中表达,而且ZaWRKY2、ZaWRKY10、ZaWRKY28这3个基因的表达量随着皮刺发育过程出现逐渐增长的现象,表明这3个基因可能参与调节皮刺的生长发育。本研究首次鉴定竹叶花椒WRKY转录因子家族结构特征和表达特性,这将为进一步解析竹叶花椒WRKY转录因子的功能提供研究基础。
WRKY is an important transcription factor family,which is widely involved in the regulation of growth and development as well as various stress responses in plants.Based on the transcriptome sequencing data from different tissue(including root,bud,aculeus and fruit)of Zanthoxylum armatum DC.,this study acquired WRKY gene sequences via the comparison of transcriptome data by local BLAST.Combined with gene expression data from different tissue,the WRKY transcription factor which is in connection with the growth and development of Z.armatum were analysed via bioinformatics analysis.Forty-three WRKY transcription factor were identified from Z.armatum transcriptome,which included eight typeⅠgenes,seven typeⅡ-a genes,eight typeⅡ-b genes,three typeⅡ-c,six typeⅡ-d,seven typeⅡ-e and four typeⅢgenes.The gene expression heat map showed that ZaWRKY8only expressed in bud,while ZaWRKY25,ZaWRKY26,ZaWRKY22,ZaWRKY29,ZaWRKY37,ZaWRKY41 only expressed in root,ZaWRKY15,ZaWRKY30 only expressed in fruit,and ZaWRKY1,ZaWRKY2,ZaWRKY10,ZaWRKY18,ZaWRKY28,ZaWRKY36,ZaWRKY43 only expressed in prickle.Besides,with the development of prickle,the expression levels of ZaWRKY2,ZaWRKY10 and ZaWRKY28 increased gradually,which revealed that these three genes might take part in the regulation of development process of aculeus.This study identified both structural characteristics and expression characteristics of WRKY transcription factor family for the first time,providing a scientific basis for further analysis of the functions of WRKY transcription factor.
作者
冯发玉
王毅
陆斌
何家妮
原晓龙
Feng Fayu;Wang Yi;Lu Bin;He Jiani;Yuan Xiaolong(College of Forestry,Southwest Forestry University,Kunming,650224;Laboratory of Forest Plant Cultivation and Utilization,The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration,Yunnan Academy of Forestry&Grassland Science,Kunming,650201;Experimental Center Tropical Forestry,Chinese Academy of Forestry,Pingxiang,532600)
出处
《分子植物育种》
CAS
北大核心
2022年第18期5984-5994,共11页
Molecular Plant Breeding
基金
云南省核桃产业关键技术创新团队项目(2018BB008)
分子生物学研究团队项目(LKYTD-2020-001)共同资助。
关键词
WRKY
竹叶花椒
转录组
生物信息学
基因表达
WRKY
Zanthoxylum armatum DC.
Transcriptome
Bioinformatics
Gene expression
