摘要
目的 研究掌叶大黄Rheum palmatum L.新鲜叶柄的化学成分。方法 采用正相硅胶柱色谱、半制备高效液相色谱、重结晶等方法进行分离和纯化,采用核磁共振波谱方法鉴定结构。结果 从掌叶大黄新鲜叶柄汁的乙酸乙酯及正丁醇萃取部位共分离纯化得到了7个化合物,分别鉴定为:β-谷甾醇(Ⅰ)、大黄素(Ⅱ)、丁二酸(Ⅲ)、原儿茶酸甲酯(Ⅳ)、原儿茶酸(Ⅴ)、槲皮素(Ⅵ)和2-羟基-丁二酸-1-甲酯(Ⅶ)。结论 化合物Ⅲ、Ⅳ、Ⅶ均为首次从掌叶大黄中分离得到;化合物Ⅳ、Ⅶ为首次从大黄属植物中分离得到。
OBJECTIVE To investigate the chemical constituents in the fresh petiole of Rheum palmatum L.METHODS The silica gel column chromatography, semi-preparative high performance liquid chromatography and recrystallization methods were used for the isolation and the purification of compounds, and the spectroscopic methods(~1HNMR,CNMR) were used for the structural identify.RESULTS Seven compounds were isolated from ethyl acetate and n-butanol extract of fresh petiole juice of Rheum palmatum L.and identified as β-sitosterol(Ⅰ),emodin(Ⅱ),succinic acid(Ⅲ),methyl 3,4-dihydroxybenzoate(Ⅳ),protocatechuic acid(Ⅴ),quercetin(Ⅵ) and 2-hydroxyl-butanedioic acid 1-methyl ester(Ⅶ).CONCLUSION Compounds Ⅲ,Ⅳ and Ⅶ have been isolated from R.palmatum,and compounds Ⅳ and Ⅶ from Rheum for the first time.
作者
张文广
冯银平
贾春艳
王丹
张云鹤
强宇靖
杨锡仓
李芸
ZHANG Wenguang;FENG Yinping;JIA Chunyan;WANG Dan;ZHANG Yunhe;QIANG Yujing;YANG Xicang;LI Yun(Gansu University of Chinese Medicine,Lanzhou,Gansu,730000 P.R.China;Afiliated Hospital of Gansu University of Chinese Medicine,Lanzhou,Gansu,730000P.R.China)
出处
《华西药学杂志》
CAS
CSCD
2022年第5期477-480,共4页
West China Journal of Pharmaceutical Sciences
基金
全国中药特色技术传承人才培训项目(T20184828005)
甘肃省高等学校产业支撑引导项目(2020C-26)
甘肃省中医药管理局科研课题(GZK-2018-6)
杨锡仓全国名老中医药专家传承工作室项目(202205)。
关键词
掌叶大黄
新鲜叶柄
廖科
半制备高效液相色谱
大黄素
黄酮类
有机酸及其酯类
核磁共振波谱法
Rheum palmatum L.
Fresh petiole
Polygonaceae
Semi-preparative high performance liquid chromatography
Emodin
Flavonoids
Organic acids and esters
NRM chromatograph
