摘要
目的:研究胃肠道调理专用奶粉润肠通便作用及对肠道菌群的影响。方法:自制胃肠道调理专用奶粉后,以KM小鼠为实验对象,采用盐酸咯哌丁胺建立小鼠便秘模型,以小鼠一般状态、小肠推进率、排便情况、小肠组织观察为考察指标结合16S rRNA基因测序分析小鼠粪便,考察其对小鼠的润肠通便作用及肠道菌群的影响。结果:胃肠道调理专用奶粉各剂量能够显著(P<0.05)缩短小鼠首粒排便时间、显著(P<0.05)提高小鼠小肠推进率,但未对小鼠小肠杯状细胞结构造成破坏,且随着剂量的提升,奶粉的胃肠调理效果越好。胃肠道调理专用奶粉虽然没有明显改变肠道微生物的丰富度和均匀度,但是能够明显改变小鼠的肠道微生态结构组成,显著(P<0.05)增加某些益生菌Lactobacillus、Bifidobacterium的相对丰度。结论:胃肠道调理专用奶粉能够影响肠道运动,调节肠道菌群结构从而改善洛哌丁胺引起的便秘。
Objective:To study the effect of special milk powder for gastrointestinal tract conditioning on intestinal bowel and intestinal microflora.Methods:After the preparation of special milk powder for gastrointestinal tract conditioning,KM mice were used as the experimental object to establish constipation models with loperamide hydrochloride.The general state,intestinal propulsion rate,defecation situation,and observation of intestinal tissue were used as the investigation indicators,and 16S rRNA gene sequencing was used to analyze the feces of mice to investigate its effects on the bowel movement and intestinal flora of mice.Results:Each dose of special milk powder for gastrointestinal tract conditioning could shorten the time of first defecation in mice(P<0.05)and improve the small intestine thrust rate(P<0.05),but did not damage the goblet cell structure of small intestine in mice.With the increasing of dose,the gastrointestinal conditioning effect of milk powder was better.Although the gastrointestinal tract conditioning special milk powder did not significantly change the richness and evenness of intestinal microbes,it could significantly change the composition of intestinal microecological structure of mice,and significantly increase the relative abundance of some probiotics,Lactobacillus and Bifidobacterium.Conclusion:Special milk powder for gastrointestinal tract conditioning could affect intestinal movement and regulate intestinal flora structure to improve constipation induce by loperamide.
作者
许贺志翔
杨晓君
沙迪昕
麦迪乃·吾秀尔
XU Hezhixiang;YANG Xiaojun;SHA Dixin;MEDINA Wuxiuer(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《食品工业科技》
CAS
北大核心
2022年第22期144-154,共11页
Science and Technology of Food Industry
基金
新疆维吾尔自治区重大专项项目项目(2020A01001-3-2)。
关键词
胃肠道调理专用奶粉
低聚糖
洛哌丁胺
肠道菌群
肠道功能
便秘小鼠
special milk powder for gastrointestinal tract conditioning
oligosaccharides
loperamide
intestinal flora
bowel function
constipation mice
