摘要
目的了解2020年宁夏地区人群常见呼吸道病毒感染分布情况,对一株人呼吸道合胞病毒(human respiratory syncytial virus,HRSV)宁夏株进行全基因组测序,对其进行基因型别鉴定及基因进化分析。方法选取2020年宁夏新冠肺炎疫情期间具有呼吸道感染症状的480人作为研究对象,采用实时荧光定量逆转录聚合酶链反应(real time reverse transcription polymerase chain reaction,real time RT-PCR)方法对甲型流感病毒(FluA)、乙型流感病毒(FluB)、呼吸道合胞病毒(RSV)、副流感病毒(PIV)Ⅰ型、Ⅱ型、Ⅲ型、呼吸道腺病毒(RAdV)、人偏肺病毒(HMPV)等8种呼吸道病原进行检测。对筛选出的HRSV进行全基因组测序,测序结果经分子生物学软件拼接,与GenBank中相关代表株序列进行分子进化分析。结果检出呼吸道病毒51例,阳性率为10.6%,RSV检出率最高(4.0%)(19/480)。宁夏首株China/ningxia/2020-02的全基因组序列为15259 bp核苷酸,与RSV-A亚型原型株Long株的核苷酸同源性最低为80.9%,与RSV-B亚型原型株9320株的核苷酸同源性为96.0%,提示了宁夏首株HRSV为B亚型。G基因遗传进化树表明,该毒株与BA9基因型代表株聚集在同一分支上,划分为BA9基因型。结论新型冠状病毒肺炎流行期间,应加强呼吸道病毒的监测,对人呼吸道合胞病毒的全基因组测序研究,有必要连续监测本地区RSV亚型流行趋势,为预防和控制RSV感染提供指导,为筛选疫苗株提供参考。
Objective To understand the distribution of common respiratory virus infections in Ningxia in 2020,the whole genome of human respiratory syncytial virus(HRSV)Ningxia strain was sequenced,and its type identification and gene evolution were analyzed.Methods During the new crown covid-19 epidemic in NingXia,480 people with respiratory tract infection symptoms were selected as the study subjects.The real time reverse transcription polymerase chain reaction was used to detect influenza A virus(FluA),influenza B virus(FluB),respiratory syncytial virus(RSV)and parainfluenza virus typeⅠ(PIVⅠ),parainfluenza virus typeⅡ(PIVⅡ),parainfluenza virus typeⅢ(PIVⅢ),respiratory adenovirus(RAdV)and human metapneumovirus(HMPV).The whole genome of the screened HRSV was sequenced.The sequencing results were spliced by molecular biology software and analyzed with the sequences of relevant representative strains in GenBank.Results 51 cases of respiratory virus were detected,and the positive rate was 10.6%.The highest detection rate of RSV was 19 cases(4.0%)(19/480).The whole genome sequence of China/Ningxia/2020-02,the first strain in Ningxia,was 15259 bp,with the lowest nucleotide homology of 80.9%with long,the prototype strain of RSV-A subtype,and 96.0%with 9320,the prototype strain of RSV-B subtype,suggesting that the first strain of HRSV in Ningxia was subtype B.The genetic evolutionary tree of G gene showed that the strain and the representative strain of BA9 genotype clustered on the same branch and were divided into BA9 genotype.Conclusion During the epidemic period of Corona Virus Disease 2019(COVID-19),the monitoring of respiratory virus should be strengthened,and the whole genome sequencing of human respiratory syncytial virus should be studied.It is necessary to continuously monitor the epidemic trend of RSV subtypes in this region,so as to provide guidance for the prevention and control of RSV infection and reference for the screening of vaccine strains.
作者
王新宁
吴忠兰
李宗倍
吴玉灵
苏艳艳
马学平
WANG Xinning;WU Zhonglan;LI Zongbei;WU Yuling;SU Yanyan;MA Xueping(School of Public Health and Management,Ningxia Medical University,NingXia Key Laboratory of Environmental Factors and Chronic Disease Control,Yinchuan 750004,China;NingXia Center for Disease Control and Prevention,Yinchuan 750004,China)
出处
《宁夏医科大学学报》
2022年第9期886-891,912,共7页
Journal of Ningxia Medical University
基金
宁夏重点研发项目(2020BEG01001)
宁夏自治区青年拔尖人才培养工程项目(RQ0049)。
关键词
呼吸道病毒
人呼吸道合胞病毒
B亚型
基因组序列分析
respiratory virus
human respiratory syncytial virus
subtype B
genome sequence analysis
