摘要
通过酪氨酸脱羧酶(TDC)催化L-酪氨酸脱羧生成酪胺,是一种低成本、高效、绿色制备酪氨的方法。利用pBAD/His为载体在宿主细胞E.coli BL21-AI(DE3)中表达了短乳杆菌来源的TDC,构建基因工程菌E.coli BL21-AI(DE3)-tdc,并对该菌L-阿拉伯糖诱导表达的条件进行优化。结果表明:在26℃培养6 h后加入终浓度为1.0 g·L^(-1)的L-阿拉伯糖诱导表达8 h,TDC酶活达183.2 U·g^(-1);在5 L发酵罐进行放大,TDC酶活达到192 U·g^(-1),菌体最高湿重为18.2 g·L^(-1),相对于摇瓶发酵的1.92 g·L^(-1)增长了8.47倍。
L-tyrosine decarboxylation catalyzed by tyrosine decarboxylase(TDC)to produce tyramine,is a low-cost,efficient,green preparation method to produce tyrosine.The TDC derived from Lactobacillus brevis was expressed in the host cell of E.coli BL21-AI(DE3)by using the pBAD/His vector,and the genetically engineered bacteria E.coli BL21-AI(DE3)-tdc was constructed,and the conditions of L-arabinose induced expression of the strain were optimized.The results showed that the enzyme activity of TDC reached 183.2 U·g^(-1)after the induced expression with 1.0 g·L^(-1)L-arabinose at 26℃for 6 h.The TDC activity reached 192 U·g^(-1)and the maximum wet weight of bacteria was 18.2 g·L^(-1),which was 8.47 times higher than that in the shaking flask fermentation,being 1.92 g·L^(-1).
作者
罗秀针
郑金华
林燕燕
吴伟斌
LUO Xiu-zhen;ZHENG Jin-hua;LIN Yan-yan;WU Wei-bin(Zhangzhou Health Vocational College,Zhangzhou,Fujian 363000,China;Fujian Vocational College of Bioengineering,Fuzhou,Fujian 350007,China)
出处
《福建农业科技》
CAS
2022年第8期8-13,共6页
Fujian Agricultural Science and Technology
基金
福建省自然科学基金项目(2021J01348)
漳州市自然科学基金项目(ZZ2020J38)。
关键词
酪胺
酪氨酸脱羧酶
构建
短乳杆菌
优化
Tyramine
Tyrosine decarboxylase
Construction
Lactobacillus brevis
Optimization
