摘要
为比较不同提取方法得到的华贵栉孔扇贝Chlamys nobilis外套膜胶原蛋白的基础特性,分别采用酸-酶法和热水法,从外套膜中提取得到胶原蛋白,分别记为A-PSC(Cn)(acid-pepsin soluble collagen Chlamys nobilis)和HSC(Cn)(hot water soluble collagen Chlamys nobilis),并测定其氨基酸组成、分子量分布、微观结构和热变性温度等理化特性。结果表明:热水法提取胶原蛋白的提取得率高于酸-酶法;HSC(Cn)中,Gly、Glu和Pro含量较高,分别为13.27、10.45、6.19 g/100 g prot,而A-PSC(Cn)中,Glu、Gly和Asp含量较高,分别为10.16、7.92、5.77 g/100 g prot;紫外光谱和红外光谱分析显示,两种胶原蛋白均在220 nm附近有强吸收峰,且具有典型的胶原蛋白特征带(酰胺A、B、Ⅰ、Ⅱ和Ⅲ带);SDS-PAGE分析显示,两种胶原蛋白均含有γ链、β链、α_(1)链和α_(2)链,由此确定这两种胶原蛋白均为Ⅰ型胶原蛋白;扫描电镜分析显示,A-PSC(Cn)呈疏松多孔状,HSC(Cn)呈紧密层状,且HSC(Cn)具有更高的热变性温度。研究表明,通过酸-酶法和热水法提取的胶原蛋白均为Ⅰ型胶原蛋白,但二者的结构特征及其理化性质存在明显差异,A-PSC(Cn)的三螺旋结构完整且纯度较高并具有疏松多孔的特性,而HSC(Cn)的亚氨基酸含量较高,可根据研究需要选用不同提取方法开发胶原蛋白产品。
In order to compare basic properties of collagen from mantle of scallop Chlamys nobilis,collagens were extracted from the mantle of the scallop by acid-enzymatic method and hot water method,named acid-pepsin soluble collagen(A-PSC(Cn))and hot water soluble collagen(HSC(Cn)),respectively,and then the amino acid composition,molecular weight,microstructure and thermal denature-temperature of collagen were determined.Results showed that there was higher collagen extraction rate by hot water method than that by acid-pepsic method,with the maximal content of 13.27 g/100 g prot in Gly,10.45 g/100 g prot in Glu and 6.19 g/100 g prot in Pro in HSC(Cn),and 10.16 g/100 g prot in Glu,7.92 g/100 g prot in Gly and 5.77 g/100 g prot in Asp in A-PSC(Cn).UV and IR spectra showed that HSC(Cn)and A-PSC(Cn)had strong absorption peak at 220 nm,and typical characteristic bands of collagen(amide A,B,Ⅰ,ⅡandⅢ).SDS-PAGE reveald thatγchain,βchain,α_(1) chain andα_(2) chain were observed in both collagens,and were identified as typeⅠcollagen.The observation of scanning electron microscopy(SEM)found that A-PSC(Cn)was loose and porous,and that HSC(Cn)was compact and layered.It was found that HSC(Cn)possessed higher thermal denaturation temperature than A-PSC(Cn)did,indicating that both HSC(Cn)and A-PSC(Cn)were all typeⅠcollagen,and there were differences in its structural characteristics and physical and chemical properties.A-PSC(Cn)has a complete triple helical structure,high purity,loose and porous characteristics,and HSC(Cn)has a high content of amino acid.The findings indicate that different extraction methods can be used to develop collagen products from scallop Chlamys nobilis according to research needs.
作者
王雯
冯畅
林海生
秦小明
曹文红
章超桦
高加龙
郑惠娜
陈忆宾
WANG Wen;FENG Chang;LIN Haisheng;QIN Xiaoming;CAO Wenhong;ZHANG Chaohua;GAO Jialong;ZHENG Huina;CHEN Yibin(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution,National Research and Development Branch Center for Shellfish Processing(Zhanjiang),South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center,Guangdong Ocean University,Zhanjiang 524088,China;Hainan Shengmeinuo Biotechnology Company Limited,Chengmai 571925,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2022年第5期858-865,共8页
Journal of Dalian Ocean University
基金
广东海洋大学博士启动基金(R17082)
现代农业产业技术体系专项资金资助(CARS-49)
广东省现代农业产业技术体系创新团队项目(2021 KJ146)
广东海洋大学贝类高值化加工利用创新团队(CXTD2021004)。
关键词
华贵栉孔扇贝
外套膜
胶原蛋白
理化性质
Chlamys nobilis
mantle
collagen
physical and chemical property
