摘要
薄壳山核桃是著名的干果和木本油料树种,当前存在“同名异物、同物异名”现象影响产业发展。本研究在薄壳山核桃基因组中筛选到80个简单重复序列(SSR),利用8个品种的基因组DNA,对80对SSR引物进行筛选,初筛到23对目标条带清晰、检测结果稳定的引物,发现它们分布于13条染色体上。进一步在Pawnee、Mahan、Stuart等36个常用品种中开展基因分型,共检测到70个等位位点;其中18对引物具有遗传多态性,检测到的等位基因数介于2~8,多态性信息含量(PIC)介于0.03~0.72。聚类分析发现,各个样本按照遗传背景聚类,不同品种之间遗传距离介于0.026~0.636,同一品种的不同来源个体之间遗传距离介于0~0.0127。按PIC值从大到小的顺序,依次增加引物的数量,直至能够区分每个品种,发现最少可使用Ciz91、Ciz85、Ciz81、Ciz140和Ciz107等5个标记实现所有品种的有效区分,进而将这组引物确定为核心引物组。最后,使用核心引物组分别构建每个品种特异的分子指纹图谱、分子身份证条形码和二维码。本研究结果为薄壳山核桃品种鉴别、种苗纯度检测、品种追溯等提供了理论基础。
Pecan[Carya illinoinensis(Wangenh.)K. Koch]is an important woody oil plant and a famous nut tree species,which has been widely cultivated in China. Adequate identification of different cultivars is showing the top priority in marketing. This study aimed to develop SSR markers which would be used for analyzing the pecan genetic diversity and constructing the molecular fingerprint and IDs. A total of 80 primer pairs were used for analyzing the polymorphisms in eight cultivars. Twenty-three(28.75%)SSR primer pairs from 13 chromosomes could amplify the target fragments. These primers were further used for genotyping in 45samples of 36 pecan cultivars,such as Pawnee,Mahan,Stuart,Kanza and Shawnee,and produced a total of 70alleles in all samples. Eighteen primer pairs were detected with polymorphisms in different cultivars,showing the number of alleles(NA)ranged from 2 to 8,and the polymorphism information content(PIC)values from 0.03to 0.72. The genetic distance between different cultivars ranged from 0.026 to 0.636,and the genetic distance between samples from different sources of the same cultivar ranged from 0 to 0.0127. A dendrogram generated by the UPGMA method suggested three groups,of which group I contained 32 cultivars and most of them have the genetic backgrounds of Schley,Success,and Major. Group Ⅱ and Group Ⅲ contained three and one cultivars,respectively. By use of(at least)five SSR primer pairs,including Ciz91,Ciz85,Ciz81,Ciz140,and Ciz107,all the cultivars were classified. These primers were selected as core SSR markers to construct molecular fingerprints and IDs. The molecular IDs were illustrated as bar codes and QR codes. Collectively,this study provided markers applicable for the cultivar identification and traceability in pecan,which has implication in the progress of pecan cultivation and production.
作者
张成才
王亚萍
王开良
常君
任华东
姚小华
ZHANG Cheng-cai;WANG Ya-ping;WANG Kai-liang;CHANG Jun;REN Hua-dong;YAO Xiao-hua(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2022年第6期1835-1846,共12页
Journal of Plant Genetic Resources
基金
国家自然科学基金青年项目(31800575)。
关键词
薄壳山核桃
品种鉴定
分子标记
SSR
指纹图谱
分子身份证
Carya illinoinensis
cultivar identification
molecular marker
SSR
DNA fingerprints
molecular ID
