摘要
为探索海绵动物体内微生物对海洋石油烃污染的生物修复作用,以石油烃降解率为指标从海绵共附生微生物中筛选降烃菌株,优选3种菌株进行16S rDNA扩增子测序鉴定和复配分析,选取最佳组合开展转速、初始pH值、温度和石油烃浓度单因素试验,对降烃效果影响较大的3种因素进行响应面优化,分析3因素对石油烃降解率的交互效应,并构建石油污染模型验证生物修复石油污染的效果.结果表明:筛选降烃菌共23株,其中降烃效果最好的Y2、Y5和Y6菌株分别鉴定为Stappia conradae、Halomonas aquamarina和Sphingopyxis terrae,其降解率均在30%以上;三种菌株复配后优选最佳组合为Y5-Y6;影响降烃效果较大的3因素为初始pH值、温度和石油烃浓度,对其响应面优化后得出最佳降解条件分别为8.12、30.55℃、17.14 g/L;按最优条件构建的模型实际降解率为50.73%,其预测值为52.52%,两者吻合度较好.该研究可为海绵-降烃菌系共生体的构建提供理论支撑,为解决海洋石油烃污染物难以集中处理的问题开辟新途径.
In order to explore the bioremediation effect of microorganisms in sponges on marine petroleum hydrocarbon pollution,hydrocarbon-reducing strains were screened from sponge-associated microorganism based on petroleum hydrocarbon degradation rate.And 3 strains were selected for 16S rDNA amplicon sequencing identification and composite analysis.The best combination was selected to carry out the single-factor test of rotational speed,initial pH value,temperature and concentration of crude oil.Three factors that have a greater impact on the hydrocarbon reduction effect were selected for response surface optimization,and analyzing the interaction effects of the three factors on the degradation rate of petroleum hydrocarbons.A model was constructed to verify the effect of bioremediation of oil pollution.The results indicated that 23 hydrocarbon-reducing bacteria strains were screened.Among them,Y2,Y5 and Y6 with the best hydrocarbon-reducing effect were identified as Stappia conradae,Halomonas aquamarina and Sphingopyxis terrae respectively,and their degradation rates were all above 30%.The best combination is Y5-Y6;and the three factors that affect the hydrocarbon reduction effect are the initial pH value,temperature and concentration of crude oil.After optimizing the response surface,the optimal degradation conditions are 8.12、30.55℃、17.14 g/L.The actual degradation rate of the model constructed according to the optimal conditions was 50.73%,and the predicted value was 52.52%,which were in good agreement.This study can provide theoretical support for the construction of sponge-hydrocarbon-depressing bacteria symbionts,and open up a new way to solve the problem of difficult centralized treatment of marine petroleum hydrocarbon pollutants.
作者
侯亚楠
刘书伟
王燕
张田田
王阿文
沈梦霞
HOU Ya-nan;LIU Shu-wei;WANG Yan;ZHANG Tian-tian;WANG A-wen;SHEN Meng-xia(College of Ecology and Environment,Hainan Tropical Ocean University,Sanya 572022,China)
出处
《陕西科技大学学报》
北大核心
2022年第6期36-42,共7页
Journal of Shaanxi University of Science & Technology
基金
海南省自然科学基金项目(221MS049,319QN240,321RC589)
海南省三亚市农业科技创新项目(2019NK12)
海南热带海洋学院研究生创新项目(RHDYC-202114,RHDYC-202121)。
关键词
石油污染
海绵动物
生物降解
响应面
菌株复配
oil pollution
sponge
biodegradation
response surface
strain compounding
