摘要
目的研究经典名方桃核承气汤化瘀泻热的“性-效”相关物质基础。方法建立蓄血证大鼠模型,考察桃核承气汤全方(17.29 g/kg)及其药性拆分成分[苦寒组(大黄+芒硝)9.43 g/kg、辛温组(桂枝+桃仁)4.71 g/kg、甘平组(甘草)3.14 g/kg]对大鼠凝血指标(凝血酶原时间、凝血酶原活动度、活化部分凝血活酶时间、纤维蛋白原、凝血酶时间、内皮素1)和炎症指标(C反应蛋白、肿瘤坏死因子α、超氧化物歧化酶)的影响。在确定优势药性组的基础上,采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法鉴定其成分,进行分子对接并筛选结合能≤-5.0 kcal/mol的成分作为“性-效”相关物质基础。结果桃核承气汤全方和辛温组药材均能显著改善模型大鼠的凝血指标(P<0.05或P<0.01),其全方和苦寒组药材均能显著改善模型大鼠的炎症指标(P<0.05或P<0.01)。辛温组为化瘀优势药性组,苦寒组为泻热优势药性组。分别从辛温组、苦寒组药材中鉴定出32、35个成分;结合能≤-5.0 kcal/mol的辛温组药材成分有10个(脱落酸、羟基酪醇、原花青素B1等),苦寒组药材成分有13个(黄芩素、芦荟大黄素、去甲蟛蜞菊内酯等)。结论桃核承气汤全方有化瘀、泻热的功效,其物质基础分别来自辛温组药材(桂枝、桃仁)和苦寒组药材(大黄、芒硝)。
OBJECTIVE To study the“property-effect”correlation material basis of the classic famous prescription Taohe chengqi decoction in removing blood stasis and purging heat.METHODS The rat model of blood storage syndrome was established to investigate the effects of Taohe chengqi decoction(17.29 g/kg)and its property grouping components[bitter-cold property group(Rhei Radix et Rhizoma+Natrii Sulfas)9.43 g/kg,pungent-warm property group(Cinnamomi Ramulus+Persicae Semen)4.71 g/kg,sweet-flat property group(Glycyrrhizae Radix et Rhizoma)3.14 g/kg]on coagulation indexes(prothrombin time,prothrombin activity,activated partial thrombin time,fibrinogen,thrombin time and endothelin-1)and inflammation indicators(Creactive protein,tumor necrosis factor-αand superoxide dismutase)in rats.On the basis of determining the dominant property groups,ultra-performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry was used to identify the components.The molecular docking was performed,and the components with binding energy≤-5.0 kcal/mol were selected as the basis of“property-effect”correlation material basis.RESULTS Taohe chengqi decoction total prescription group and pungent-warm property group could significantly improve the coagulation indexes of model rats(P<0.05 or P<0.01),and the total prescription group and bitter-cold property group could significantly improve the inflammation indexes of model rats(P<0.05 or P<0.01).The pungent-warm property group was the dominant property group for removing blood stasis,and the bitter-cold property group was the dominant property group for purging heat.Thirty-two and thirty-five components were identified from the pungentwarm property group and bitter-cold property group,respectively.With binding energy≤-5.0 kcal/mol,there were 10 components(abscisic acid,3,4-dihydroxyphenylethanol,procyanidin B1,etc.)in the pungent-warm property group and 13 components(baicalein,aloe-emodin,demethylwedelolactone,etc.)in the bitter-cold property group.CONCLUSIONS Taohe chengqi decoction has the effect of removing blood stasis and purging heat,and its material basis comes from pungent-warm property group(Cinnamomi Ramulus,Persicae Semen)and bitter-cold property group(Rhei Radix et Rhizoma,Natrii Sulfas)respectively.
作者
舒万芬
刘文
宋信莉
刘兴德
张甘纯
秦琴
王洪鑫
SHU Wanfen;LIU Wen;SONG Xinli;LIU Xingde;ZHANG Ganchun;QIN Qin;WANG Hongxin(School of Pharmacy,Guizhou University of Traditional Chinses Medicine,Guiyang 550025,China;School of Pharmacy,Guizhou Medical University,Guiyang 550025,China)
出处
《中国药房》
CAS
北大核心
2022年第22期2706-2711,共6页
China Pharmacy
基金
国家自然科学基金资助项目(No.82060704,No.81860706)
贵州省特色功能食品与中药制剂开发集成攻关项目(No.黔教合KY字〔2020〕006)
贵州中医药大学药用高分子材料研究中心项目(No.贵中医党办发〔2019〕70号)。
关键词
桃核承气汤
蓄血证
药性
凝血指标
炎症指标
物质基础
大鼠
Taohe chengqi decoction
blood storage syndrome
medical property
coagulation index
inflammatory indicator
material basis
rats
