摘要
建立了一种基于纸芯片的黄酮类化合物含量快速检测新方法.黄酮类化合物与NaNO_(2)-Al(NO_(3))_(3)-NaOH在纸芯片上反应显红色,采用手机拍照记录结果,PhSp_CS2软件分析色度值.ρ(黄酮类化合物)=0.0588~0.2940mg/mL,显色强度RGB值和ρ(芦丁)呈良好的线性关系.该方法日内重复性为1.44%,日间重复性为1.71%,平均加标回收率为99.44%.采用该方法对刺蒺藜提取液ρ(总黄酮)进行测定,与分光光度法检测结果符合较好.该方法快速、准确、简单,适用于ρ(总黄酮)测定.
A new method for rapid determination of flavonoids was developed based on a microfluidic paper-based analytical device through colorimetric analysis.Here,NaOH is loaded in the round paper chip firstly.Flavonoids react with Al^(3+)that subsequently changes in base to form the red complex.The changes in color intensity were recorded using a common cell phone and subsequently analyzed using PhSp_CS2 software.A linear regression of the color intensity RGB and the rutin concentration was obtained with a linear range of 0.0588 to 0.2940 mg/mL,and relative standard deviation(RSD)of 1.44%(inter-day)and 1.71%(intra-day)for paper chips.The effects of detection conditions have been investigated and are discussed.The extracst of Tribulus terrestris samples were detected.The results obtained are consistent with the spectrophotometry method.The work demonstrates the potential of this method as a low sample cost and rapid colorimetric technique to detect flavonoids.
作者
张剑
郑志鸿
张博
张宁宁
唐新萍
李一冰
夏敏珠
刘春叶
ZHANG Jian;ZHENG Zhihong;ZHANG Bo;ZHANG Ningning;TANG Xinping;LI Yibing;XIA Minzhu;LIU Chunye(School of Pharmacy,Xi'an Medical University,Xi'an 710021,China)
出处
《化工科技》
CAS
2022年第4期66-69,共4页
Science & Technology in Chemical Industry
基金
陕西省科技厅社发项目(2021SF-323)
国家级大学生创新创业训练计划项目(202111840003,121521003)
陕西省科技厅自然科学基金面上项目(2020JM-614)
陕西省教育厅服务地方科学研究计划项目(19JC035)
西安医学院校内人才项目(2018XNRC03)。
关键词
微流控纸芯片
黄酮类化合物
含量测定
刺蒺藜
比色法
Microfluidic paper chip
Flavonoid
Content determination
Tribulus terrestris
Colorimetric method
