摘要
玉米秸秆中粗纤维含量达33.4%,纤维素降解后制备的还原糖可作为发酵工业的碳源。为了提高纤维素的降解效率,以风干后的玉米秸秆为主要原料,还原糖得率为评价指标,采用微波-超声波辅助、稀硫酸和纤维素酶协同作用降解玉米秸秆中的纤维素,通过单因素实验和正交实验优化降解工艺。分别分析了微波时间、超声波时间、稀硫酸浓度、纤维素酶添加比例和纤维素酶反应时间对纤维素降解的影响。实验结果表明:纤维素酶添加比例28 mg/g、酶解时长3 h、超声波处理时长40 min、微波处理时长60 s、稀硫酸浓度为2%时秸秆纤维素降解效果最好,还原糖得率为37.8%。
The content of crude fiber in corn stover is 33.4%,and the reducing sugar prepared after cellulose degradation can be used as a carbon source for fermentation industry.In order to improve the degradation efficiency of cellulose,air-dried corn stover was used as the main raw material,and the reduction sugar yield was used as the evaluation index.Microwave-ultrasound assisted,dilute sulfuric acid and cellulase synergistic effect were used to degrade cellulose in corn stover.The effects of microwave time,ultrasonic time,dilute sulfuric acid concentration,cellulase addition ratio and cellulase reaction time on cellulose degradation were analyzed by single factor experiment and orthogonal experiment.The experimental results showed that the cellulose degradation effect of straw was the best when the cellulase addition ratio was 28 mg/g,the enzymatic hydrolysis time was 3 h,the ultrasonic treatment time was 40 min,the microwave treatment time was 60 s,and the dilute sulfuric acid concentration was 2%,and the reduction sugar yield was 37.8%.
作者
刘俊红
王建成
王福梅
洪军
LIU Jun-hong;WANG Jian-cheng;WANG Fu-mei;HONG Jun(School of Life Science&Engineering,Henan University of Urban Construction,Pingdingshan 467036,China)
出处
《河南城建学院学报》
CAS
2022年第5期80-86,共7页
Journal of Henan University of Urban Construction
基金
河南省科技厅基础与前沿项目(092300410083)。
关键词
微波
超声波
纤维素酶
还原糖
microwave
ultrasound
cellulase
reducing sugar
