摘要
目的建立红景天苷血浆蛋白结合率测定方法,比较常氧及缺氧大鼠血浆中红景天苷蛋白结合率的差异。方法采用低压低氧动物实验舱(海拔7500 m)制备缺氧大鼠模型,采用超滤法分离血浆中游离型药物,高效液相色谱法分别测定常氧及缺氧大鼠血浆中药物浓度,并计算常氧及缺氧大鼠血浆中红景天苷的蛋白结合率。色谱柱为Thermo C18,流动相为乙腈(A)-0.1%甲酸水(B),梯度洗脱:0~16 min,5%~8%A,16~20 min,5%A,流速:1.0 ml·min^(-1),检测波长:275 nm,柱温:30℃,进样量:15μl。结果当大鼠血浆中红景天苷浓度为3、6、12μg·ml^(-1)时,红景天苷与常氧大鼠血浆的蛋白结合率分别为(18.34±4.33)%、(18.84±4.45)%和(19.25±2.98)%;与缺氧大鼠血浆的蛋白结合率分别为(12.19±2.78)%、(11.74±4.34)%和(11.47±1.50)%,其平均血浆蛋白结合率降低7.01%,两者比较差异有统计学意义(P<0.05或P<0.01)。结论红景天苷与常氧大鼠血浆蛋白属于低强度结合,在本研究的浓度范围内,不具浓度依赖性。相比于常氧大鼠,红景天苷与缺氧大鼠血浆蛋白的结合强度有小幅度的降低。
Objective To establish a method for determining the plasma protein binding rate of salidroside,and study the difference in this rate between normoxic and hypoxic rats.Methods A hypoxic rat model was established via a hypobaric hypoxia animal experiment cabin(at an altitude of 7500 m).The ultrafiltration method was employed to separate free drugs in plasma while HPLC was used to determine the concentration of salidroside in the plasma of normoxic and hypoxic rats.The plasma protein binding rate of salidroside was calculated.The analytical column was Thermo C18.Mobile phase A consisted of acetonitrile while mobile phase B was composed of formic acid aqueous solution(0.1%)by gradient elution.The detection wavelength was 275 nm.The flow rate was 1.0 ml·min^(-1).The column temperature was 30℃and the sample volume was 15μl.Results When the concentration of salidroside in rat plasma was 3,6 and 12μg·ml^(-1),the plasma protein binding rates of salidroside among normoxic rats were(18.34±4.33)%,(18.84±4.45)%and(19.25±2.98)%respectively,compared with(12.19±2.78)%,(11.74±4.34)%and(11.47±1.50)%for hypoxic rats.The difference in the plasma protein binding rate was sig-nificant between normoxic and hypoxic rats(P<0.05 or P<0.01).Conclusion The intensity of plasma protein binding of salidroside in normoxic rats is low,and there is concentration-independence within the concentration range used in this study.The binding intensity of salidroside to plasma protein in hypoxic rats is obviously lower than in normoxic rats.
作者
王维刚
李晓琳
王俊丽
刘天龙
王芃
李茂星
WANG Wei-gang;LI Xiao-lin;WANG Jun-li;LIU Tian-long;WANG Peng;LI Mao-xing(School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China;The 940th Hospital of Joint Logistic Support Force of PLA,Lanzhou 730050,China;Drug and Instrument Supervisory and Test Station,Xining Joint Logistics Support Center,Lanzhou 730050,China)
出处
《解放军药学学报》
CAS
2022年第3期193-197,共5页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
甘肃省重点研发项目(20YF3FA035)
甘肃省卫生行业科研计划项目(GSWSKY2020-41)
青年培育项目(2021yxky060)
中央高校基本科研业务费专项资金项目(31920200010)
应急医学科研项目(20yjky018)。
