摘要
炎症是生物体内与许多疾病相关的重要保护反应,过氧亚硝酸根(ONOO-)参与炎症过程,与炎症有着密切联系。采用6-羟基-2,3,4,4a-四氢-1H-氧杂蒽-1-酮(GCTPOC)作为双光子荧光团(双光子荧光活性截面δΦ=262 GM(1 GM=1×10^(-50)cm^(4)·s·photons^(-1)·molecule^(-1)),激发波长λ_(ex)=860 nm)设计并合成一种用于检测过氧亚硝酸根的双光子荧光探针GCTONOO。该探针具有良好的荧光响应,检出限低(13.5 nmol/L)、Stokes位移大(135 nm),可特异性识别ONOO^(-)。细胞急性炎症模型实验结果表明,该探针可实现细胞炎症过程中ONOO^(-)的荧光成像分析。
Inflammation is an important protection reaction in living organisms associated with many diseases. Peroxynitrite(ONOO-) is involved in the inflammatory processes and has an important relationship with inflammation. Herein, a two-photon fluorescence probe GCT-ONOO for detecting peroxynitrite was designed and synthesized using 6-hydroxy-2,3,4, 4a-tetrahydro-1H-oxanthracene-1-one(GCTPOC) as the two-photon fluorophore(two-photon fluorescence activity cross section δΦ=262 GM(1 GM=1×10^(-50)cm^(4)·s·photons^(-1)·molecule^(-1)),excitation wavelength λ_(ex)=860 nm). The probe can specifically recognize ONOO^(-) with good fluorescence response, low detection limit(13.5 nmol/L) and large Stokes displacement(135 nm). The results of cell experiment in acute inflammation model showed that the probe can achieve highly sensitive imaging analysis for peroxynitrite during cellular inflammation.
作者
王旭梅
高明昱
孙琦
宋新建
WANG Xumei;GAO Mingyu;SUN Qi;SONG Xinjian(Hubei Key Laboratory of Biological Resources Protection and Utilization,Hubei Minzu University,Enshi 445000,Hubei,China;School of Chemical and Environmental Engineering,Hubei Minzu University,Enshi 445000,Hubei,China)
出处
《武汉大学学报（理学版）》
CAS
CSCD
北大核心
2022年第4期397-404,共8页
Journal of Wuhan University:Natural Science Edition
基金
国家自然科学基金地区科学基金(22166017)
湖北省重点实验室开放基金(PT012003)。
