摘要
为制备达氟沙星(DAN)多克隆抗体,建立检测DAN残留的间接竞争ELISA(ic-ELISA)方法,采用混合酸酐法制备免疫原DAN-BSA和包被原DAN-OVA,用DAN-BSA免疫新西兰大白兔获得DAN多抗血清,建立检测DAN的ic-ELISA检测方法。结果显示,成功合成了DAN-BSA和DAN-OVA,并获得DAN的多克隆抗体;用该抗体建立的ic-ELISA检测方法的半数抑制浓度为0.39μg/L,最低检测限为0.04μg/L,检测范围为0.09μg/L~6.16μg/L。结果表明,成功制备了抗DAN的多克隆抗体,并建立了检测DAN的ic-ELISA方法,为进一步开发DAN快速检测试剂盒奠定了基础。
To prepare danofloxacin(DAN)polyclonal antibodies and establish an ic-ELISA method for detecting DAN residue,the immunogen DAN-BSA and the coating original DAN-OVA were prepared by the mixed acid anhydride method.The polyclonal antibodies were obtained by immunizing New Zealand white rabbits with DAN-BSA,and an ic-ELISA to detect DAN was established.The results showed that DAN-BSA and DAN-OVA were successfully synthesized,and DAN polyclonal antibodies were successfully obtained;The half inhibitory concentration of the ic-ELISA method is 0.39μg/L,the lowest detection limit is 0.04μg/L,and the detection range is 0.09μg/L-6.16μg/L.In summary,the ic-ELISA method was established with polyclonal antibodies for detecting DAN,which laid the foundation for the further development of DAN rapid detection kit.
作者
张欣欣
齐永华
岳锋
路少鹏
李文明
郭东光
潘鹏涛
王选年
ZHANG Xin-xin;QI Yong-hua;YUE Feng;LU Shao-peng;LI Wen-ming;GUO Dong-guang;PAN Peng-tao;WANG Xuan-nian(Henan Engineering Laboratory for Molecular Diagnosis of Animal Diseases,Xinxiang University,Xinxiang,Henan,453000,China;Zhengzhou University,Zhengzhou,Henan,450000,China)
出处
《动物医学进展》
北大核心
2022年第12期84-89,共6页
Progress In Veterinary Medicine
基金
国家重点研发计划项目(2018YFC1602900)。
