摘要
Direct messenger ribonucleic acid(mRNA)delivery to target cells or tissues has revolutionized the field of biotechnology.However,the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded nanocarriers.Herein,we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells(hADMSCs)via electrically controlled mRNA delivery.To find optimal electrical conductivity and mRNAloading capacity,the polypyrrole-graphene oxide(PPy-GO)hybrid film was electropolymerized on indium tin oxide substrates.We found that the fluorescein sodium salt,a molecule partially mimicking the physical and chemical properties of mRNAs,can be effectively absorbed and released by electrical stimulation(ES).The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical stimulation.This platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.
基金
supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(Nos.NRF-2019M3A9H_(2)031820,NRF-2021R1A2C1010747,and NRF-2022R1A2C4002217)
Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT,and Ministry of Health and Welfare(Grant No.RS-2022-00070316)
the Bio&Medical Technology Development Program funded by the Ministry of Science,ICT and Future Planning,Republic of Korea(NRF-2017M3A9E4047243)
d K.S.also thankful for The Council of Higher Education(CoHE,100/2000)PhD Scholarship Program,Turkey.
