摘要
目的建立反相高效液相色谱法测定95%乙醇、75%乙醇、55%乙醇、35%乙醇、15%乙醇、水、正丁醇、甲醇8种不同提取溶剂白花蛇舌草中芦丁、槲皮素、山柰酚3种黄酮类化合物的含量并加以比较,优选出最佳提取溶剂。方法采用Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇(B)-0.1%磷酸(D),梯度洗脱,检测波长为365 nm,流速0.8 m L·min^(-1),柱温30℃,进样量10μL,同时进行方法学验证。结果芦丁、槲皮素、山柰酚分别在0.06~0.72μg(R~2=1)、0.0235~0.282μg(R~2=0.9991)、0.17~2.04μg(R~2=0.9998)范围内线性关系良好;平均加样回收率(n=6)分别为96.81%、99.90%、102.03%,RSD分别为0.63%、3.36%、0.64%。以75%乙醇为溶剂提出的芦丁、槲皮素含量最高,山柰酚含量以95%乙醇溶剂提取最高。结论不同溶剂提取对白花蛇舌草中芦丁、槲皮素、山柰酚的含量影响很大。
OBJECTIVE To establish and compare the contents of rutin,quercetin and kaempferol flavonoids in Hedyotis Diffusa with eight different extraction solvents,including 95% ethanol,75% ethanol,55% ethanol,35%ethanol,15% ethanol,water,n-butanol and methanol by RP-HPLC,and to select the best extraction solvent.METHODS An Agilent ZORBAX SB-C_(18) column( 4.6 mm × 250 mm,5 μm) was used with the mobile phase of methanol( B)-0.1% phosphoric acid( D) and gradient elution with the detection wavelength of 365 nm,flow rate of 0.8 m L·min^(-1),column temperature of 30 ℃,injection volume of 10 μL,and simultaneous methodological validation.RESULTS The linearity ranges of rutin,quercetin and kaempferol fell into 0.06-0.72 μg( R~2= 1),0.0235-0.282 μg( R~2= 0,9991) and 0.17-2.04 μg( R~2= 0.9998) respectively;The average spiked recoveries of rutin,quercetin and kaempferol were 96.81%,99.90%,102.03% with RSD 0.63%,3.36%,0.64% respectively.As The highest content of rutin and quercetin was presented with 75% ethanol as the solvent,and the highest content of kaempferol was extracted with 95% ethanol solvent.CONCLUSION Different solvent extracts had a great effect on the contents of rutin,quercetin and kaempferol in Hedyotis Diffusa.
作者
龙小妹
林艾和
李蓉
夏宗霄
郭爽
范源
LONG Xiao-mei;LIN Ai-he;LI Rong;XIA Zong-xiao;GUO Shuang;FAN Yuan(Yunnan University of Chinese Medicine,Kunming 650500,China;Second Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650216,China)
出处
《海峡药学》
2022年第10期30-34,共5页
Strait Pharmaceutical Journal
基金
云南省高校中西医结合防治甲状腺病重点实验室(31170101803)
云南省万人计划“名医”专项。
