摘要
目的研究基于Toll样受体4(TLR4)/核因子κB(NF-κB)通路探究益气活血清热解毒方对动脉粥样硬化模型大鼠的干预效果。方法将60只大鼠随机分为正常组10只、其余50只大鼠建立动脉粥样硬化模型作为动脉粥样硬化组,喂养4周后每组随机抽样2只,取主动脉经HE染色光镜观察,以发现动脉粥样硬化斑块为建模成功,建模成功后将动脉粥样硬化组剩余48只大鼠分为模型组(9只)、辛伐他汀组(9只)、益气活血组(10只)、清热解毒组(10只)、益气活血清热解毒中药组(10只)5个亚组。建模成功后益气活血组、清热解毒组、益气活血清热解毒中药组分别给予药液9 ml/(kg·d)体重灌胃,辛伐他汀片组给予辛伐他汀药液5 ml/(kg·d)体重灌胃,正常组和模型组给予生理盐水9 ml/(kg·d),以上各组均连续灌胃8周。对比分析各组的血脂指标甘油三脂(TG)、血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白(HDL-C)、超敏C反应蛋白(Hs-CRP)、白介素-6(IL-6)水平及TLR4/NF-κB通路蛋白表达量。结果与正常组相比,模型组、辛伐他汀组、益气活血组、清热解毒组、益气活血清热解毒中药组TG、TC、LDL-C、Hs-CRP、IL-6水平升高,HDL-C水平降低,差异具有统计学意义(P<0.05);与模型组相比,辛伐他汀组、益气活血组、清热解毒组、益气活血清热解毒中药组TG、TC、LDL-C、Hs-CRP、IL-6水平降低,HDL-C水平升高,差异具有统计学意义(P<0.05);与辛伐他汀组、益气活血组、清热解毒组相比,益气活血清热解毒中药组TG、TC、LDL-C、Hs-CRP、IL-6水平降低,HDL-C水平升高,差异具有统计学意义(P<0.05)。与模型组相比,益气活血清热解毒中药组TLR4、NF-κB表达量降低(P=0.001)。结论益气活血清热解毒方可起到调节动脉粥样硬化大鼠血脂、缓解炎症症状的作用,其机制可能与抑制TLR4/NF-κB通路有关。
Objective To explore the interventional effect of Yiqi Huoxue Qingre Jiedu(Qi-tonifying,blood circulation-activating,heat-clearing,and detoxification)recipe on atherosclerosis rats based on the TLR4/NF-κB pathway.Methods Sixty rats were randomly divided into either a normal group(10 rats)or an atherosclerosis group(50 rats;atherosclerosis was induced).After feeding for 4 weeks,two rats were randomly selected from each group,and the aorta was dissected and observed under a light microscope after HE staining to find atherosclerotic plaque.After successful modeling,the remaining 48 rats in the atherosclerosis group were divided into five groups:model group(9 rats),simvastatin group(9 rats),Qi-tonifying and blood circulation-activating group(10 rats),heat-clearing and detoxification group(10 rats),and Qi-tonifying,blood circulation-activating,heat-clearing,and detoxification group(10 rats).The Qi tonifying and blood circulation-activating group,the heat-clearing and detoxification group,and the Qi activating,blood circulation-activating,heat-clearing,and detoxification group were given the corresponding traditional Chinese medicine recipes at 9 ml/kg/d body weight,respectively;the simvastatin tablet group was given 5 mg/(kg·d)body weight of simvastatin solution,and the normal group and the model group were given 9 ml/kg/d of normal saline.All the treatments were given intragastrically for 8 consecutive weeks.The levels of TG,TC,LDL-C,HDL-C,hs-CRP,and IL-6 and the protein expression of TLR4 and NFκB in different groups were compared and analyzed.Results Compared with the normal group,the levels of TG,TC,LDL-C,hs-CRP,and IL-6 were significantly increased in the model group,simvastatin group,Qi-tonifying and blood circulation-activating group,heat-clearing and detoxification group,and Qi-tonifying,blood circulation-activating,heat-clearing,and detoxification groups,while the level of HDL-C was significantly decreased(P<0.05).Compared with the model group,the levels of TG,TC,LDL-C,hs-CRP,and IL-6 were significantly decreased in the simvastatin group,Qi-tonifying and blood circulation-activating group,heat-clearing and detoxification group,and Qi-tonifying,blood circulation-activating,heat-clearing,and detoxification group,while the level of HDL-C was significantly increased(P<0.05).Compared with the simvastatin group,Qi-tonifying and blood circulation-activating group,and heat-clearing and detoxification group,the levels of TG,TC,LDL-C,hs-CRP,and IL-6 in the Qi-tonifying,blood circulation activating,,heat-clearing,and detoxification group were significantly decreased,while the level of HDL-C was significantly increased(P<0.05).Compared with the model group,the protein expression of TLR4 and NFκB was significantly reduced in the Qi-tonifying,blood circulation-activating,,heat-clearing,and detoxification group(P=0.001).Conclusion Yiqi Huoxue Qingre Jiedu recipe can regulate blood lipids and relieve inflammatory symptoms in atherosclerotic rats via mechanisms that may be related to the inhibition of the TLR4/NF-κB pathway.
作者
刘志勇
任德启
郭健
赵彦青
杨明辉
李锋森
成家宏
Liu Zhiyong;Ren Deqi;Guo Jian;Zhao Yanqing;Yang Minghui;Li Fengsen;Cheng Jiahong(Department of Encephalopathy,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Department of Encephalopathy,Henan Provincial Hospital of Traditional Chinese Medicine,Zhengzhou 450002,China)
出处
《中华临床医师杂志（电子版）》
CAS
北大核心
2022年第4期349-355,共7页
Chinese Journal of Clinicians(Electronic Edition)
基金
王松龄全国名老中医药专家工作室(国中医药人教发2018134号)
河南省中医药科学研究专项重点课题(20-21ZY1024)
河南省中医药拔尖人才培养项目(豫中医科教201835号)
河南省中医院中青年学术骨干(35-45)培育。
