摘要
目的 探究菟丝子黄酮(FSC)对核因子E2相关因子2(Nrf2)/血红素加氧酶-1(HO-1)通路的调控作用以及对精索静脉曲张(VC)大鼠生精功能的影响。方法 90只雄性SD大鼠随机分为假手术组、模型组、迈之灵组、FSC低、中、高剂量组,并建立VC大鼠模型。实验结束肾静脉取血,检测血清中丙二醛(MDA)含量与超氧化物歧化酶(SOD)活性;取左侧附睾分析精子浓度与精子活力;取睾丸观察组织形态变化和细胞凋亡情况,检测一氧化氮(NO)含量以及Nrf2、HO-1蛋白表达。结果 与假手术组比较,模型组大鼠睾丸组织结构病变明显,生精小管上皮变薄,出现变形甚至塌陷,生精细胞缺失,管腔内精子数量明显变少;血清中MDA含量[模型组、假手术组分别为(1.86±0.12)nmol/mL、(0.42±0.06)nmol/L]、生精细胞凋亡指数[模型组、假手术组分别为(26.50±1.89)%、(5.33±0.51)%]、NO含量[模型组、假手术组分别为(1.63±0.10)mg/mL、(0.88±0.08)mg/mL]明显升高(P<0.05),SOD活性、精子浓度与精子活力以及Nrf2、HO-1蛋白表达均明显降低(P<0.05)。与模型组比较,FSC各剂量组大鼠睾丸组织结构病变明显改善,血清中MDA含量、生精细胞凋亡指数、NO含量明显降低(P<0.05),SOD活性、精子浓度与精子活力以及Nrf2、HO-1蛋白表达均明显升高(P<0.05),且呈剂量依赖性(P<0.05)。与迈之灵组比较,FSC低、中剂量组大鼠睾丸组织结构病变较明显,上述指标变化差异有统计学意义(P<0.05);FSC高剂量组差异无统计学意义(P>0.05)。结论 FSC可以通过激活睾丸组织中Nrf2/HO-1信号通路,抗氧化应激、抗细胞凋亡,保护VC大鼠生精功能。
Objective To explore the regulation of flavonoids from Semen Cuscutae(FSC)on nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)pathway and the effect on spermatogenesis in rats with varicocele(VC).Methods Ninety male SD rats were randomly divided into sham operation group,model group,Aescuven forte group,FSC low,medium and high dose groups. Except for the sham operation group,the other five groups established VC rat models.After the experiment,the renal venous blood of rats in each group was taken to detect the MDA content and SOD activity in the serum;after the rats were sacrificed,the left epididymis was taken to analyze the sperm concentration and sperm motility;the testis was taken,the changes in tissue morphology and cell apoptosis were observed,and the content of nitric oxide(NO)and the expression of related proteins in the Nrf2/HO-1 pathway were detected. Results Compared with the sham operation group,the testicular tissue structure of the model group had obvious pathological changes,the seminiferous tubule epithelium became thin,deformed or even collapsed,spermatogenic cells were missing,and the number of sperm in the lumen was significantly reduced;the serum MDA content[(1.86±0.12)nmol/mL and(0.42±0.06)nmol/mL,respectively],spermatogenic cell apoptosis index[(26.50±1.89)% and(5.33±0.51)%,respectively],and NO content[(1.63±0.10)mg/mL and(0.88±0.08)mg/mL,respectively]in testicular tissue were significantly increased(P<0.05),and the SOD activity,sperm concentration,sperm motility,and the expression of Nrf2 and HO-1 protein were significantly reduced(P<0.05).Compared with the model group,the testicular tissue structure pathology of rats in each FSC dose group was significantly improved,the serum MDA content,spermatogenic cell apoptosis index,and NO content in testicular tissue were significantly reduced(P<0.05),and the SOD activity,sperm concentration,sperm motility,and the expression of Nrf2 and HO-1 protein were significantly increased(P<0.05),and were dose-dependent(P<0.05). Compared with the Aescuven forte group,the testicular tissue structure of rats in the FSC low-and medium-dose groups had more obvious pathological changes,the serum MDA content,spermatogenic cell apoptosis index,and NO content in testicular tissue were significantly increased(P<0.05),and the SOD activity,sperm concentration,sperm motility,and the expression of Nrf2 and HO-1 protein were significantly reduced(P<0.05);there was no significant difference in the above indicators in the FSC high-dose group(P>0.05).Conclusion FSC can activate the Nrf2/HO-1 signaling pathway in the testis to play anti-oxidative stress damage and antiapoptosis effects,and protect the spermatogenic function of VC rats.
作者
郭石磊
张丽云
李志强
张云
吴真富
Guo Shi-lei;Zhang Li-yun;Li Zhi-qiang;Zhang Yun;Wu Zhen-fu(Department of Urology,Ningde hospital,Fujian 352100)
出处
《解剖学研究》
CAS
2022年第5期419-424,共6页
Anatomy Research
