摘要
目的通过网络药理学方法分析香薷(Moslae Herba)治疗流感潜在活性成分、靶点以及作用机制。方法从中药系统药理学数据库与分析平台(TCMSP)中获取香薷化学成分,利用SwissTargetPrediction数据库得到化合物潜在靶点,通过GeneCards、OMIM数据库和文献挖掘获取流感靶点;利用Cytoscape软件构建成分靶点网络图,通过STRING数据库获取交集靶点的蛋白质相互作用(PPI)并导入Cytoscape软件,采用Metascape平台对交集靶点进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析。结果共获得香薷14个活性成分及其243个潜在靶点,流感相关靶点1088个,香薷成分和流感交集靶点45个;研究发现香薷治疗流感的有效活性化合物为14个,关键成分为柯伊利素、木犀草素、山奈酚、华良姜素、金合欢素和槲皮素等6个;PPI网络分析得关键靶点蛋白9个,主要为Akt丝氨酸/苏氨酸激酶1(Akt1)、非受体酪氨酸激酶(SRC)、热休克蛋白(HSP)90AA1、表皮生长因子受体(EGFR)、基质金属蛋白酶9(MMP9)、前列腺素内过氧化物合酶2(PTGS2)、雷帕霉素靶蛋白(mTOR)等;Metascape基因富集分析筛选得到GO条目总计708条,主要与细胞因子、免疫调节、氧化应激等多个生物学过程相关;筛选发现KEGG 130条通路,主要涉及病原体感染以及磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路、趋化因子信号通路、磷脂酶D信号通路等。结论该研究揭示香薷可能通过抗炎、抗病毒、免疫调节等机制治疗流感,为后续实验研究提供一定参考。
Objective To analyze the potential active ingredients,targets and mechanism of anti-influenza effect of Moslae Herba using network pharmacology methods.Methods The chemical composition of Moslae Herba was retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The potential targets of compounds were collected from the SwissTargetPrediction database,and the influenza targets were retrieved by GeneCards,OMIM database and literature mining.The ingredients-targets′network was constructed with Cytoscape,and the protein-protein interactions(PPIs)of intersection targets were obtained from the STRING database and imported into Cytoscape.The intersection targets′enrichment analysis of gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)was carried out by Metascape.Results Fourteen active ingredients and 243 potential targets of Moslae Herba were found and 1088 influenza-related targets were obtained.There were 45 targets where the components of the Moslae Herba and influenza intersected.There were 14 effective active compounds of Moslae Herba that were effective influenza,among which the six key ingredients were chryseriol,luteolin,kaempferol,kumatakenin,acacetin and quercetin.PPI network analysis resulted in nine critical target proteins,including Akt1,SRC,HSP90AA1 and EGFR.Metascape gene enrichment analysis resulted in 708 GO terms,which were related to multiple biological processes such as cytokines,immunomodulation and oxidative stress.One hundred and thirty KEGG pathways were also obtained through gene enrichment analysis,involving pathogen infection,PI3K-Akt signaling pathway,chemokine signaling pathway and phospholipase D signaling pathway.Conclusion This study reveals that Moslae Herba may help treat influenza via its anti-inflammatory,antiviral and immunomodulatory proporties,which provides valuable reference for future experimental studies.
作者
金雅晴
祖勉
洪永凯
李丽娟
JIN Ya-qing;ZU Mian;HONG Yong-kai;LI Li-juan(Institute of Health Service and Transfusion Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)
出处
《军事医学》
CAS
2022年第9期691-698,共8页
Military Medical Sciences
关键词
香薷
流感
网络药理学
中药药理
Moslae Herba
influenza
network pharmacology
pharmacology of traditional Chinese materia medica
