摘要
目的观察参蛤散对H9c2心肌细胞增殖的影响,为参蛤散后续研究提供依据。方法采用H9c2心肌细胞株,制备参蛤散冻干粉进行干预,在24 h、48 h、72 h各时间点,用细胞计数试剂盒-8(CCK-8)法检测细胞吸光度值(OD值),细胞划痕实验检测细胞迁移率。结果24 h参蛤散0.03 mg/mL、0.10 mg/mL、0.30 mg/mL、1.00 mg/mL组OD值高于对照组(P<0.05);48 h参蛤散各浓度组OD值均高于对照组(P<0.05);72 h参蛤散0.01 mg/mL组OD值高于对照组(P<0.05),其余组与对照组比较差异均无统计学意义(P>0.05)。24 h参蛤散0.10 mg/mL、0.30 mg/mL、1.00 mg/mL组细胞迁移率大于对照组(P<0.05);48 h参蛤散0.01 mg/mL、0.10 mg/mL、0.30 mg/mL组细胞迁移率大于对照组(P<0.05);72 h参蛤散0.01 mg/mL、0.30 mg/mL组细胞迁移率大于对照组(P<0.05)。结论参蛤散可以促进H9c2心肌细胞增殖。
Objective To explore the effect of Shen′ge Powder(SGP)on H9c2 cardiomyocyte proliferation.Methods The freeze-dried powder of SGP was prepared for intervention of H9c2 cardiomyocyte.Optical density(OD)value was detected by CCK-8 assay,and cell mobility was detected by cell wound healing assay at 24 h,48 h,and 72 h.Results Compared with control group,OD value was increased in SGP 0.03 mg/mL,0.10 mg/mL,0.30 mg/mL,and 1.00 mg/mL groups at 24 h(P<0.05),in all SGP groups at 48 h(P<0.05),and in SGP 0.01 mg/mL group at 72 h(P<0.05).Compared with control group,cell migration rate was increased in SGP 0.10 mg/mL,0.30 mg/mL,and 1.00 mg/mL groups at 24 h(P<0.05),in SGP 0.01 mg/mL,0.10 mg/mL,and 0.30 mg/mL groups at 48 h(P<0.05),and in SGP 0.01 mg/mL and 0.30 mg/mL groups at 72 h(P<0.05).Conclusion SGP can promote H9c2 cardiomyocyte proliferation.
作者
邱伯雍
魏易洪
苑素云
毛美娇
曹敏
周端
邓兵
沈琳
QIU Boyong;WEI Yihong;YUAN Suyun;MAO Meijiao;CAO Min;ZHOU Duan;DENG Bing;SHEN Lin(First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450000,Henan,China)
出处
《中西医结合心脑血管病杂志》
2022年第22期4098-4101,共4页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
国家自然科学基金资助项目(No.81804010)
上海中医药大学附属龙华国家中医临床研究基地“龙医育苗”计划(No.LYTD-83)
上海中医药大学高水平大学建设经费(No.A1-U1820501030201)。
关键词
H9C2心肌细胞
增殖
参蛤散
实验研究
H9c2 cardiomyocyte
proliferation
Shen′ge Powder
experiment research
