摘要
为提高木聚糖的酶解效果,试验在筛选出最佳产木糖效率的GH11家族木聚糖酶Xyn1B基础上,分析瘤胃真菌新丽鞭毛菌来源GH11木聚糖酶Xyn1B和黑曲霉源GH10家族木聚糖酶Xyn4的酶解特性,探究木聚糖酶GH11 Xyn1B和木聚糖酶GH10 Xyn4配伍使用对糖产量及产物中寡糖组成的影响。试验中使用的GH10 Xyn4和GH11 Xyn1B单一木聚糖酶及其不同配伍混合木聚糖酶溶液的酶活力浓度均为0.879 IU/mL,然后测定木聚糖酶的种类、添加量以及GH10 Xyn4和GH11 Xyn1B木聚糖酶不同配伍比例对木聚糖酶解效果的影响。结果表明,在哈茨木霉GH11敏感木聚糖酶XynHar、瘤胃真菌新丽鞭毛菌GH11抗性木聚糖酶Q9U、瘤胃真菌新丽鞭毛菌GH11抗性木聚糖酶Xyn1B中,抗性木聚糖酶GH11 Xyn1B的催化效率最高,所得酶解产物的OD值最高;GH11 Xyn1B在酶用量为400μL时产糖量接近峰值;GH11木聚糖酶Xyn1B和GH10木聚糖酶Xyn4的复配酶液催化产糖量均高于单独酶液,反应时间2 h时、GH11 Xyn1B:GH10 Xyn4为5:5时产糖量可以达到最高,酶解产物中包含较高含量的木二糖、木三糖以及其他木寡糖。
In order to improve the enzymatic hydrolysis of xylan, based on the screened xylanase Xyn1B with the best xylose-producing efficiency in CH11 family, the enzymatic characteristics of Aspergillus niger Xyn4 GH10 xylanase and Neocallimastix patriciarum GH11 Xyn1B xylanases were analyzed in this experiment, and the effects of GH11 xylanase Xyn1B and GH10 xylanase Xyn4 on sugar production and oligosaccharide composition in the product was explored. The activities of single or mixed GH11 xylanase Xyn1B and GH10 xylanase Xyn4 in this experiment were adjusted to the same size(0.879 IU/mL).Then the effects of xylanase species, addition amount and different Xyn1B: Xyn4 compatibility ratio on xylanase hydrolysis were determined. The results showed that, among Trichoderma harziana GH11 sensitive xylanase XynHar and rumen fungi Neocallimastix patriciarum resistant xylanase Q9U and Xyn1B,the resistant-inhibitor xylanase GH11 Xyn1B had the highest catalytic efficiency and OD value. The sugar yield of GH11 Xyn1B was close to the peakwhen the enzyme dosage was 400 μL. The sugarconcentration produced by the compound enzymeof GH11 xylanase Xyn1B and GH10 xylanaseXyn4 was higher than that of the single xylanase.The sugar yield could reach the highest whenXyn1B:Xyn4 was 5:5 at two hours of reaction.The enzymatic hydrolysis products contained high content of xylobiose, xylotriose and other xylo-oligosaccharides.
作者
姬慧鑫
付涵芬
陈晓艺
陈红歌
刘新育
JI Huixin;FU Hanfen;CHEN Xiaoyi;CHEN Hongge;LIU Xinyu(College of Life Sciences,Henan Agricultural University,Key Laboratory of Enzyme Engineering of Agricultural Microbiology,Ministry of Agriculture and Rural Affairs,Henan Zhengzhou 450002,China)
出处
《饲料工业》
CAS
北大核心
2022年第23期29-32,共4页
Feed Industry
基金
NSFC-河南人才培养联合基金[U1404324]
河南教育厅科学技术研究重点项目[21A180008]。
