摘要
目的:分析本实验室儿童促甲状腺激素(TSH)、甲状腺素(T4)、三碘甲状腺素(T3)、游离甲状腺素(FT4)、游离三碘甲状腺原氨酸(FT3)、黄体生成素(LH)、促卵泡生成素(FSH)、孕酮(PROG)、泌乳素(PRL)、雌二醇(E2)和睾酮(T)的性别、年龄分布规律,探索其生物参考区间。方法:从2018年8月至2021年12月来温州医科大学附属第二医院儿童保健科体检的人群中筛选出1~18岁的健康个体,按幼儿前男、女组(1~3岁),幼儿女组(4~8岁)、幼儿男组(4~10岁),青少年女组(9~18岁)、青少年男组(11~18岁)分成6组;剔除离群值,获合格例数783例。采用SIEMENS ADVIA Centaur XP System化学发光分析仪测定其血清激素水平,组间比较采用t检验、近似t检验或Kruskal-Wallis检验,相关性分析采用Spearman分析。结果:FT3、LH、FSH、PRL、E2、T水平在不同年龄组男女间差异有统计学意义(P<0.05)。男童T3、T4与年龄呈负相关(r=-0.419、-0.347,P<0.05),LH、FSH、PROG、T与年龄表现出较强正相关(r=0.768、0.678、0.535、0.703,P<0.05);女童T3、T4、FT3与年龄呈负相关(r=-0.486、-0.318、-0.424,P<0.05),LH、FSH、PROG、E2、T与年龄呈正相关性(r=0.811、0.335、0.574、0.584、0.744,P<0.05)。青少年男、女组中Tanner分期与LH、FSH、PRL、PROG、E2、T的相关系数分别为0.641、0.204、0.308、0.479、0.604、0.686和0.664、0.399、0.436、0.485、0.621、0.649,均呈正相关(P<0.01)。基于各年龄组不同性别儿童的甲状腺激素和性激素水平,初步拟定温州地区1~18岁儿童11项激素的参考区间。结论:本研究对儿童甲状腺激素和性激素11项水平的初步调查结果和目前本科室所使用的欧洲参考区间之间存在差异,建立本地区的参考区间具有临床意义。
Objective:To explore the biological reference interval of children in Wenzhou area by analyzing their gender and age distribution of thyrotropin(TSH),thyroxine(T4),3,5,3’triiodothyronine(T3),free thyroxine(FT4),free triiodothyroxine(FT3),luteinizing hormone(LH),follicle stimulating hormone(FSH),progesterone(PROG),prolactin(PRL),estradiol(E2)and testosterone(T).Methods:Healthy children aged 1-18 years were selected from those who underwent physical examination between August 2018 and December 2021 in the Children’s Health Care Department of the Second Affiliated Hospital of Wenzhou Medical University.They were divided into 6 groups as 1-3 years old(male and female group),4-8 years old(female group),4-10 years old(male group),9-18 years old(female group)and 11-18 years old(male group).With excluding outliers,783 qualified cases were obtained.The serum hormone level was measured by SIEMENS ADVIA Centaur XP System chemiluminescence analyzer.The comparison between groups was analyzed by t test,approximate t test or Kruskal-Wallis test,and the correlation s analyzed by Spearman.Results:The levels of FT3,LH,FSH,PRL,E2 and T were significantly different between boy and girl in different age groups(P<0.05).In boys,age was negatively correlated with T3,T4(r=-0.419,-0.347,P<0.05),while it showed strong positive correlation with LH,FSH,PROG,T(r=0.768,0.678,0.535,0.703,P<0.05).Girls’age was negatively correlated with T3,T4,FT3(r=-0.486,-0.318,-0.424,P<0.05),while it was positively correlated with LH,FSH,PROG,E2,T(r=0.811,0.335,0.574,0.584,0.744,P<0.05).The correlation coefficients of Tanner stage and LH,FSH,PRL,PROG,E2,T in adolescent male and female groups were 0.641,0.204,0.308,0.479,0.604,0.686 and 0.664,0.399,0.436,0.485,0.621,0.649 respectively.It’s positive correlation(P<0.01).Based on the thyroid hormone and sex hormone levels of children of different age and gender groups,the reference intervals of 11 hormones for children aged 1-18 in Wenzhou area were preliminarily formulated.Conclusion:There’re differences in the level of thyroid and sex hormones in children between the findings of this study and the European reference intervals currently used in our department,which is of clinical significance in terms of regional reference intervals.
作者
李姗姗
倪莉
徐晓杰
朱丽丹
丁红香
LI Shanshan;NI Li;XU Xiaojie;ZHU Lidan;DING Hongxiang(Laboratory Medicine Center,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China)
出处
《温州医科大学学报》
CAS
2022年第12期965-971,共7页
Journal of Wenzhou Medical University
关键词
甲状腺激素
性激素
参考区间
儿童
thyroid hormone
sex hormones
reference interval
children
