摘要
目的:分析重楼2种基原植物(云南重楼和七叶一枝花)3个部位(根茎、须根、芽)中9个甾体皂苷(重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅲ、重楼皂苷Ⅳ、重楼皂苷Ⅴ、重楼皂苷Ⅵ、重楼皂苷Ⅶ、重楼皂苷H、重楼皂苷D)含量的差异。方法:运用高效液相色谱法(HPLC)测定9个皂苷的含量;通过SPSS 26.0软件对9个成分含量之间的相关性进行分析;通过Origin 9.8插件Heat Map Dendrogram对重楼不同基原、不同部位的9个皂苷含量进行聚类分析;应用SIMCA 13.0软件进行主成分分析。结果:重楼中薯蓣皂苷类和偏诺皂苷类成分的含量在不同基原、不同部位间比较均有一定程度的差异,其中云南重楼根茎中的薯蓣皂苷含量显著高于七叶一枝花根茎,而其偏诺皂苷含量显著低于七叶一枝花根茎,2种基原的3个部位也具有一定程度的差异。结论:重楼不同基原植物中9个重楼皂苷含量存在差异,临床上应考虑将其分开使用;须根和芽的存在会影响根茎中皂苷的含量和比例,炮制时应将其去除以保证药物质量;须根和芽也含有较多重楼皂苷,可以将其作为单一皂苷提取原料以加强资源综合利用。研究结果为重楼的合理使用和不同部位的充分利用提供参考。
Objective:To analyze the differences in the content of nine saponins(polyphyllinⅠ,polyphyllinⅡ,polyphyllinⅢ,polyphyllinⅣ,polyphyllinⅤ,polyphyllinⅥ,polyphyllinⅦ,polyphyllin D,and polyphyllin H).in three parts(rhizome,fibrous root,and bud)of two origin plants of Paridis Rhizoma,i.e.,Paris polyphylla Smith var.yunnanensis(Franch.)Hand.-Mazz and Paris polyphylla Smith var.chinensis(Franch.)Hara.Methods:The content of nine saponins was determined by HPLC.The correlations of the content of the nine saponins were analyzed by SPSS 26.0.The content of nine saponins in different parts and origin plants of Paridis Rhizoma was analyzed by Origin 9.8 plug-in Heat Map Dendrogram.SIMCA 13.0 was used for principal component analysis(PCA).Results:The content of dioscin and pennogenin was different in different parts and the two origin plants.The content of dioscin in the P.polyphylla Smith var.yunnanensis was significantly higher than that in P.polyphylla Smith var.chinensis,while the content of pennogenin was significantly lower.Besides,such deviation could be observed in three parts of the two origin plants.Conclusion:The two origin plants of Paridis Rhizoma should be used separately in clinical practice due to the different content of nine types of polyphyllin.The fibrous root and bud should be cut from the main root when using clinically,because they can affect the content and proportion of saponins in the main root.The fibrous root and bud also contain multiple polyphyllin,which can be used as raw materials for extracting single saponin to enhance the comprehensive utilization of resources.The research results are expected to provide some references for the rational use of Paridis Rhizoma and the full use of its different parts.
作者
王旭星
贾天颖
杨成金
文佳
徐新房
苏钛
叶先文
程水清
王璇
李向日
WANG Xu-xing;JIA Tian-ying;YANG Cheng-jin;WEN Jia;XU Xin-fang;SU Tai;YE Xian-wen;CHENG Shui-qing;WANG Xuan;LI Xiang-ri(Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica,Centre of Traditional Chinese Medicine Processing Research,Beijing University of Chinese Medicine,Beijing 102488,China;Tongrentang Pharmaceutical Factory,Beijing Tongrentang Co.,Ltd.,Beijing 102629,China;Yunnan Baiyao Group Chinese Medicinal Resources Co.,Ltd.,Kunming 650500,China)
出处
《中国现代中药》
CAS
2022年第11期2132-2140,共9页
Modern Chinese Medicine
基金
国家中药标准化项目(ZYBZH-Y-YN-44)
国家药典委员会标准制修订研究课题(2018Z006)
北京中医药大学重点攻关项目(2020-JYB-ZDGG-035)。
关键词
云南重楼
七叶一枝花
重楼皂苷
根茎
须根
芽
Paris polyphylla Smith var.yunnanensis(Franch.)Hand.-Mazz
Paris polyphylla Smith var.chinensis(Franch.)Hara
polyphyllin
rhizome
fibrous root
bud
