摘要
目的分析孕妇叶酸代谢关键基因位点多态性的分布情况及其与早产的关系。方法回顾性分析221例于2020年1月至2021年1月在南京医科大学附属泰州人民医院建立孕检档案并在孕早期做叶酸代谢关键基因筛查者的临床资料。根据孕妇分娩结果将研究对象分为早产组(n=125)和正常分娩组(n=96)。采用金磁微粒层析法检测2组受试者的叶酸代谢关键基因5,10-亚甲基四氢叶酸还原酶(MTHFR)C677T位点基因多态性。比较2组受试者一般资料及基因型分布情况。采用多因素logistic回归分析影响孕妇发生早产的危险因素。结果早产组孕妇的年龄、体质量指数、文化程度、孕前3个月配偶是否吸烟、孕前3个月配偶是否酗酒、家庭经济情况、工作、孕次、自然流产史及人工流产史等情况与正常分娩组比较差异无统计学意义(P>0.05)。早产组中居住在乡村、有妊娠高血压综合征、通过人工辅助生殖技术怀孕、孕前补充叶酸时间为1个月及有孕期服药史者所占比例显著高于正常分娩组(P<0.05);早产组受试者的叶酸水平显著低于正常分娩组(P<0.05)。早产组与正常分娩组受试者MTHFR基因C677T位点基因型和等位基因的分布频率比较差异有统计学意义(P<0.05)。多因素logistic回归分析结果显示,MTHFR基因C677T位点CC基因型及等位基因T的分布频率是孕妇发生早产的危险因素(P<0.05)。结论孕妇叶酸代谢关键基因MTHFR C677T位点基因型及等位基因分布频率是孕妇发生早产的危险因素。
Objective To analyze the distribution of polymorphism of key gene locus of folic acid metabolism in pregnant women and its relationship with premature delivery.Methods The clinical data of 221 pregnant who were established with pregnancy examination files in Taizhou People′s Hospital Affiliated to Nanjing Medical University from January 2020 to January 2021 and screened for key genes of folic acid metabolism in early pregnancy were analyzed retrospectively.The pregnant women were divided into premature delivery group(n=125)and normal delivery group(n=96)according to the delivery results.The general data and genotype distribution of subjects in the two groups were compared.The gene polymorphism of 5,10-methylenetetrahy drofolate reductase(MTHFR)C677T locus in the key gene of folic acid metabolism of subjects in the two groups was detected.The risk factors of premature delivery in pregnant women were analyzed by multivariate logistic regression.Results There was no significant difference in the age,body mass index,education level,whether the spouse smoked in the first three months of pregnancy,whether the spouse drank excessively in the first three months of pregnancy,family economic situation,whether the pregnant woman work,pregnancy times,history of spontaneous abortion and history of induced abortion between the preterm delivery group and the normal delivery group(P>0.05).The proportions of living in the countryside,having pregnancy-induced hypertension syndrome,being pregnant through artificial assisted reproductive technology,taking folic acid for one month before pregnancy and taking medicine during pregnancy in the premature delivery group were significantly higher than those in the normal delivery group(P<0.05);the level of folic acid of subjects in the premature delivery group was significantly lower than that in the normal delivery group(P<0.05).There was significant difference in the distribution frequency of genotype and allele of MTHFR gene C677T locus between the preterm delivery group and the normal delivery group(P<0.05).Multivariate logistic regression analysis showed that the distribution frequency of CC genotype and allele T at C677T locus of MTHFR gene were risk factors for premature delivery in pregnant women(P<0.05).Conclusion The distribution of genotype and allele of MTHFR gene C677T locus of key genes of folic acid metabolism in pregnant women are the risk factor for premature delivery in pregnant women.
作者
杜濛濛
金春燕
顾志萍
DU Mengmeng;JIN Chunyan;GU Zhiping(Department of Medical Genetics and Prenatal Diagnosis,Taizhou People′s Hospital Affiliated to Nanjing Medical University,Taizhou 225300,Jiangsu Province,China)
出处
《新乡医学院学报》
CAS
2022年第12期1168-1172,共5页
Journal of Xinxiang Medical University
关键词
早产
基因多态性
叶酸代谢关键基因
preterm birth
gene polymorphism
key gene of folic acid metabolism
