摘要
目的探讨精浆微小核糖核酸-34c(miR-34c)的表达与少精症、弱精症患者精液质量的临床关系。方法选取2020年1月至2022年3月浙江省丽水市人民医院收治的135例少精症患者为少精症组,156例弱精症患者为弱精症组,同期选取150例育龄期健康男性作为对照组。采用实时荧光定量聚合酶链反应技术检测精浆miR-34c表达水平,比较三组的精浆miR-34c表达水平和精子质量参数(包括精液量、精子总数、精子浓度、精子活力a+b级百分比、精子前向运动率),采用Pearson相关性分析少精症、弱精症患者精浆miR-34c表达水平与精液质量的关系。结果三组的精浆miR-34c表达水平比较,差异有统计学意义(P<0.05);少精症组、弱精症组的miR-34c表达水平均低于对照组(P<0.05);少精症组与弱精症组比较,差异无统计学意义(P>0.05);三组的精液量比较,差异无统计学意义(P>0.05);三组的精子总数、精子浓度、精子活力a+b级百分比、精子前向运动率比较,差异有统计学意义(P<0.05);少精症组、弱精症组以上指标均低于对照组(P<0.05);少精症组患者的精子总数、精子浓度低于弱精症组(P<0.05);少精症组患者的精子活力a+b级百分比、精子前向运动率高于弱精症组(P<0.05);Pearson相关分析结果显示,少精症组、弱精症组患者的精浆miR-34c表达水平与精液量无相关性(P>0.05),与精子总数、精子浓度、精子活力a+b级百分比及精子前向运动率呈正相关(P<0.05)。结论少精症、弱精症患者的精浆miR-34c表达水平均低于健康育龄期男性,且与其精液质量均呈正相关,miR-34c表达水平越高,精子总数越多,精子浓度、精子活力a+b级百分比及精子前向运动率越高。
Objective To investigate the clinical relationship between the expression of microribonucleic acid-34c(miR-34c)in seminal plasma and semen quality in patients with oligozoospermia and asthenospermia.Methods A total of 135 patients with oligozoospermia treated in Lishui People's Hospital of Zhejiang Province from January 2020 to March 2022 were selected as oligozoospermiagroup,a total of 156 asthenospermia patients as asthenospermia group,and 150 healthy men of childbearing age were selected as control group at the same time.The expression level of miR-34c in seminal plasma was detected by real-time fluorescence quantitative polymerase chain reaction.The expression level of miR-34c in seminal plasma and sperm quality parameters(included semen volume,total sperm count,sperm concentration,percentage of a+b grade of sperm activity,sperm forward movement rate)were compared among the three groups.The relationship between the expression level of miR-34c in seminal plasma and semen quality in patients with oligozoospermia and asthenospermia was analyzed by Pearson correlation analysis.Results The expression level of miR-34c in seminal plasma of the three groups was statistically significant(P<0.05).The comparison of total sperm count,sperm concentration,percentage of sperm activity a+b and sperm forward movement rate among the three groups showed significant difference(P<0.05).The above indicators in oligozoospermia group and asthenospermia group were lower than those in the control group(P<0.05).The total sperm count and sperm concentration in oligozoospermia group were lower than those in asthenospermia group(P<0.05).The percentage of sperm activity a+b and sperm forward movement rate in oligozoospermia group were higher than those in asthenospermia group(P<0.05);Pearson correlation analysis showed that the expression level of miR-34c in seminal plasma of patients with oligozoospermia and asthenospermia was not correlated with semen volume(P>0.05),but was positively correlated with total sperm count,sperm concentration,percentage of a+b grade of sperm motility and sperm forward movement rate(P<0.05).Conclusion The expression level of miR-34c in seminal plasma of patients with oligozoospermia and asthenospermia is lower than that of healthy men of childbearing age,and is positively correlated with their semen quality.
作者
余亮亮
李朋
YU Liangliang;LI Peng(Department of Urology,Lishui People's Hospital,Zhejiang,Lishui 323000,China)
出处
《中国现代医生》
2022年第33期44-47,97,共5页
China Modern Doctor
关键词
微小核糖核酸-34c
少精症
弱精症
精液质量
精浆
Microribonucleic acid-34c
Oligospermia
Asthenospermia
Semen quality
Seminal plasma
