摘要
【目的】为明确南方水稻黑条矮缩病病毒(Southern rice blackstreaked dwarf virus,SRBSDV)对抗白背飞虱水稻的抗虫性的影响。【方法】在水稻3叶期,每稻苗接入8头1-2龄白背飞虱Sogatella furcifera(Horváth)若虫,参照国际标准评价不同水稻对白背飞虱的抗性表现;每稻苗接入已饲毒且度过循回期的2头白背飞虱高龄若虫,评价不同抗性水平的抗白背飞虱水稻对SRBSDV的抗性。在各水稻成株期分别将不同虫态白背飞虱接到SRBSDV感染的稻株,研究不同抗性水平水稻感染SRBSDV后对白背飞虱取食选择性、取食量、存活率以及产卵量与卵孵化率的影响【结果】中抗、抗和高抗白背飞虱的水稻对SRBSDV的抗性均表现高感,且发病率均在93.55%以上;把无毒白背飞虱若虫分别接入各抗白背飞虱水稻的健康株和SRBSDV病株,48h后在健康株上取食的虫量为1.33-5.33头/株,而在SRBSDV病株上取食的虫量为24.00-46.00头/株,无毒白背飞虱取食选择偏向于SRBSDV病株,且其趋性与水稻对白背飞虱的抗性表现之间无明显相关性;白背飞虱成虫在各水稻健康株和感染SRBSDV的病株上取食,48h后其平均蜜露量分别为1.13-9.55 mg/头和2.97-17.35 mg/头,各抗白背飞虱水稻的感染SRBSDV病株之间白背飞虱的平均蜜露量差异较大,但均显著多于各自健康株上的平均蜜露量;白背飞虱在表现高抗和抗水平水稻上的存活率在15%-44.05%之间,而在感染SRBSDV病株上的存活率显著提高,均在69.21%以上;白背飞虱在各感染SRBSDV抗白背飞虱水稻上无毒白背飞虱能正常产卵及繁殖,且其产卵量和卵孵化率显著多于或高于各自对应健康株。【结论】抗白背飞虱的水稻不一定抗SRBSDV;抗白背飞虱水稻感染SRBSDV,有利于提高白背飞虱在取食、存活和繁殖方面对水稻抗虫性的适应度。
[Objectives]To detect the effects of the southern rice black-streaked dwarf virus(SRBSDV)on the resistance of white-backed planthopper(WBPH)resistant rice varieties to the WBPH.[Methods]81st-2nd instar WBPH nymphs were placed on individual rice seedlings to evaluate the resistance of different rice varieties to WBPH,according to the international standard.Each seedling was infected with 2 older nymphs that had been fed with the virus and had passed the cycle period to evaluate the resistance of WBPH-resistant rice varieties to SRBSDV.Different life-stages of WBPH were placed on SRBSDV-infected rice to study the effects of SRBSDV-infection of WBPH-resistant,rice varieties on the feeding selectivity,feeding capacity,survival rate,fecundity and hatchability of WBPH.[Results]Rice varieties with moderate,resistance and high resistance to WBPH were highly susceptible to SRBSDV,with an incidence rate of more than 93.55%.Non-virus WBPH nymphs were inoculated onto both healthy and SRBSDV-infected,WBPH-resistant rice plants.After 48 hours,healthy plants had 1.33-5.33 nymphs/plant whereas SRBSDV-infected plants had 24.00-46.00 nymphs/plant.WBPH prefered to feed on SRBSDV-infected plants,but there was no significant correlation with the resistance of rice to WBPH.After WBPH adults had fed on healthy and SRBSDV-infected rice plants for 48 hours,the average amount of honeydew was 1.13-9.55 mg/adult and 2.97-17.35 mg/adult,respectively.The average amount of honeydew secreted by WBPH feed on SRBSDV-infected,WBPH-resistant,rice varieties was significantly different and higher than that healthy plants.The survival rate of WBPHs on rice with resistance or high resistance was between 15%-44.05%,whereas their survival rate on plants infected with SRBSDV was significantly higher;>69.21%.The non-virus WBPH can reproduce normally on on WBPH-resistant rice varieties that are infected with SRBSDV,and egg production and egg hatching rate are significantly higher on infected plants than on healthy plants.[Conclusion]SRBSDV infection of WBPH-resistant rice improves the feeding,survival and reproduction of the WBPH.WBPH-resistant rice varieties are not necessarily resistant to SRBSDV and the resistance of such varieties is decreased by SRBSDV infection.
作者
黄所生
秦碧霞
吴碧球
谢慧婷
李成
李战彪
黄凤宽
蔡健和
HUANG Suo-Sheng;QIN Bi-Xia;WU Bi-Qiu;XIE Hui-Ting;LI Cheng;LI Zhan-Biao;HUANG Feng-Kuan;CAI Jian-He(Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs,Nanning 530007,China;Guangxi Key Laboratory for Biology of Crop Diseases and Insect Pests,Nanning 530007,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2022年第5期1048-1058,共11页
Chinese Journal of Applied Entomology
基金
广西自然科学基金项目(2018GXNSFBA281155,2021GXNSFAA196059)
国家现代农业产业技术体系广西创新团队建设项目(nycytxgxcxtd-2021-01-05)
广西农业科学院基本科研业务专项项目(桂农科2021YT073)
广西农业科学院科技发展基金项目(桂农科2017JM30,2015JZ44,2015JZ45,2015JZ46)。
关键词
南方水稻黑条矮缩病病毒
白背飞虱
水稻品种
抗虫性
取食量
southern rice black-streaked dwarf virus
Sogatella furcifera
rice variety
insect resistance
feeding amount
