摘要
目的探讨标准化制片流程在根治性前列腺癌(PC)病理诊断中的价值。方法选取2019年1月至2020年4月间宝鸡市中医医院收治的前列腺癌根治术(RP)后做病理切片的40例患者作为研究对象,根据制片流程的不同将患者分为观察组(30例)和对照组(10例)。观察组运用标准化制片流程,对照组运用传统病理制片流程。比较两组患者的优片率、重切率及切片镜下表现情况;并记录观察组病理诊断结果。结果观察组患者共有1240张病理切片,优片率为99.8%(1237/1240),重切率为0.2%(2/1240)。对照组10例患者共248张切片,优片率为81.3%(201/248),重切率为18.7%(47/248),两组优片率与重切率比较,差异均有统计学意义(均P<0.05)。两组苏木精-伊红(HE)片镜下显示,观察组HE片切厚薄更均匀,表面组织更完整、且无震颤、无褶皱、无刀痕,即切片平整度更完好;观察组病理诊断结果为:前列腺癌侵犯神经9例(63.3%),累及双侧叶16例(53.3%),局部切缘阳性4例(13.3%)。结论对前列腺根治术组织标本运用标准化流程进行HE病理制片,能提高HE片质量,提高优片率,降低重切率,且镜下显示经标准化制片流程所制作的HE片,切片厚薄更均匀,切面组织更完整,且无震颤、无褶皱、无刀痕,即切片平整度更完好。
Objective To analyze the significance of standardized sectioning processes-based pathological sectioning of hematoxylin and eosin(HE) for pathological diagnosis at radical prostatectomy for prostate cancer(PC). Methods Forty patients whose tissues were removed from the site of prostate cancer and were sectioned at radical prostatectomy(RP) at Baoji Hospital of Traditional Chinese Medicine were selected from January 2019 to April 2020. According to the different sectioning processes, the patients were divided into an observation group(30 patients) and a control group(10 patients). The observation group adopted the standardized sectioning process, and the control group adopted the traditional pathological sectioning process. The excellent film rate, recutting rate and the performance of section under the microscope were compared between the two groups. The pathological diagnosis results of the observation group were recorded. Results There were 1240 pathological sections for 30 patients in the observation group, the excellent film rate was 99.8%(1237/1240), and the recutting rate was 0.2%(2/1240). There were 248 sections for 10 patients in the control group, the excellent film rate was 81.3%(201/248), and the recutting rate was 18.7%(47/248). There were significant differences in the excellent film rate and recutting rate between the two groups(all P<0.05). Under the microscope, it showed that the cutting thickness of the HE sections was more uniform, and the surface tissue was more intact without tremors, wrinkles or scalpel marks, that is, the flatness of the slices was better in the observation group. The pathological diagnosis results for the observation group showed that prostate cancer invaded nerves in 9 patients(63.3%), involved bilateral lobes in 16 patients(53.3%), and positive local margin in 4 patients(13.3%). Conclusion Tissue specimens for pathological examination at radical prostatectomy using the standardized sectioning processes can improve the quality of HE sections and the excellent rate and reduce the recutting rate. Microscopically, it shows that the HE sections produced by the standardized sectioning process have more uniform slice thickness and more complete section tissue without tremors, wrinkles or scalpel marks, that is, the flatness of a section is more intact.
作者
王晓宝
巨宏亚
陈妮
WANG Xiao-bao;JU Hong-ya;CHEN Ni(Department of Pathology,Baoji Hospital of Traditional Chinese Medicine,Baoji 721000,China)
出处
《中国肿瘤临床与康复》
2022年第11期1328-1332,共5页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
前列腺肿瘤
根治术
病理制片
标准化制片流程
Prostate neoplasms
Radical operation
Pathological section
Standardized sectioning process
